yhxD

168

general stress protein, similar to alcohol dehydrogenase

locus: BSU_10430

Molecular weight: 31.76 kDa

pI: 4.54

Protein length: 299 aa

Gene length: 900 bp

function: survival of salt and ethanol stresses

product: unknown

essential: no

synonyms: yhxD

List of homologs in different organisms, belongs to COG1028 (Galperin et al., 2021)

Gene

Coordinates: 1,117,734  1,118,633

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The protein

Protein family

• [wiki|Short-chain dehydrogenases/reductases (SDR) family] (according to UniProt)

Structure

• [PDB|3R3S] (from ''Salmonella Enterica'', 66% identity)
• [AF|P40398]

Paralogous protein(s)

• [protein|0DD474462720CAEE2AE17017BD7CA385238EBC6F|yxbG], [protein|20A7DBA142F3BF8DCAD9732BD908CE8CC79AA993|bacC], [protein|439B468A13137000FB42E9389391CB4986FFED84|fabG]
• [protein|B6FF689E65906186F3576B378650D713DB84EDDA|ycdF], (31,1%)
• [protein|CA4597C6253CCF7D7954686A30AF041808BDF8E5|gdh], (33,5%)
• [protein|EAEEA4DD9641919830A81185333A2610B964D37C|yhdF], (47%)
• [protein|3161519994609DA9360ED6E073E4A4C8C6210EFB|ydaD], (48,8%)
• [protein|4DEFC2998464BF8327578C36A13A10DD277F991E|ykvO], (34,5%)
• [protein|6047F2493FCE3D2A9BDB1AD88E5926ECEED81036|yhxC], (45,2%),

Expression and Regulation

Operons

• genes: [gene|739B228743BC1FE9E6888E999BC0E4F615E36F9E|yhxD]

  description: [Pubmed|8196543]

  regulation

  • induced by stress ([protein|search|SigB]) [Pubmed|15805528]

  sigma factors

  • [protein|580011DE5DC40EC9E7E0512791D328FAA010DCB8|sigB]: sigma factor, [Pubmed|15805528], in [regulon|protein:580011DE5DC40EC9E7E0512791D328FAA010DCB8|sigB regulon]

  additional information

  • the mRNA is very stable (half-life > 15 min) [http://www.ncbi.nlm.nih.gov/sites/entrez/12884008 PubMed]

Biological materials

Mutant

• MGNA-A719 (yhxD::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/719 NBRP B. subtilis, Japan]
• BKE10430 ([gene|739B228743BC1FE9E6888E999BC0E4F615E36F9E|yhxD]::erm  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE10430 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CATCAGCAACGCTCCTTTTC,  downstream forward: _UP4_TAGCTTTATGAAAGGAGCTG
• BKK10430 ([gene|739B228743BC1FE9E6888E999BC0E4F615E36F9E|yhxD]::kan  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK10430 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CATCAGCAACGCTCCTTTTC,  downstream forward: _UP4_TAGCTTTATGAAAGGAGCTG

References

• 12884008,8196543,15805528