yhfP

168

putative quinone oxidoreductase, Zn-dependent and NAD(P)-binding

locus: BSU_10320

Molecular weight: 34.60 kDa

pI: 4.72

Protein length: 330 aa

Gene length: 993 bp

function: unknown

product: putative quinone oxidoreductase

essential: no

synonyms: yhfP

List of homologs in different organisms, belongs to COG0604 (Galperin et al., 2021)

Gene

Coordinates: 1,106,524 → 1,107,516

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The protein

Protein family

• [wiki|zinc-containing alcohol dehydrogenase family] (according to UniProt)

[wiki|Cofactors]

• NADP⁺ (according to UniProt)

Structure

• [PDB|1TT7] (99% identity)
• [PDB|1Y9E] (with NAD),  [PDB|1TT7]
• [AF|O07615]

[wiki|Localization]

• cytoplasm (according to Swiss-Prot)

Biological materials

Mutant

• MGNA-A715 (yhfP::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/715 NBRP B. subtilis, Japan]
• BKE10320 (Δ[gene|FE034FDCEAD4F4E91FD0147E16685632069E3316|yhfP]::erm  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE10320 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CATTCGGCACACTCCTTTTC,  downstream forward: _UP4_TAACAGGATCAGCTTGCAGA
• BKK10320 (Δ[gene|FE034FDCEAD4F4E91FD0147E16685632069E3316|yhfP]::kan  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK10320 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CATTCGGCACACTCCTTTTC,  downstream forward: _UP4_TAACAGGATCAGCTTGCAGA