yhdR

168

similar to aspartate aminotransferase

locus: BSU_09570

Molecular weight: 43.73 kDa

pI: 5.01

Protein length: 393 aa

Gene length: 1182 bp

function: unknown

product: unknown

essential: no

ec: 2.6.1.1

synonyms: yhdR

List of homologs in different organisms, belongs to COG0436 (Galperin et al., 2021)

Gene

Coordinates: 1,034,046  1,035,227

The protein

Catalyzed reaction/ biological activity

• 2-oxoglutarate + L-aspartate --> L-glutamate + oxaloacetate (according to UniProt), this activity is rather unlikely as the [gene|AE802E1D4F827DED3A5D953C7D034E6A3554C45F|aspB] mutant is auxotrophic for aspartate indicating that [protein|8F7432F7EF0F767C6000D553A3ACE29FBA2EDCEE|yhdR] does not contribute to aspartate synthesis [pubmed|29995990]

Protein family

• [wiki|class-I pyridoxal-phosphate-dependent aminotransferase family] (according to UniProt)

[wiki|Cofactors]

• PLP

Structure

• [PDB|3ELE] (from Eubacterium rectale, 34% identity)
• [AF|O07587]

[wiki|Localization]

• cytoplasm (according to Swiss-Prot)

Additional information

• The gene is annotated in KEGG as aspartate aminotransferase EC 2.6.1.1. In MetaCyc the protein is marked as similar to aspartate aminotransferase. No EC annotation is available in Swiss-Prot. No literature/experimental evidence supporting the annotation is availablavailable.  [Pubmed|19935659]

Expression and Regulation

Operons

• genes: [gene|8F7432F7EF0F767C6000D553A3ACE29FBA2EDCEE|yhdR]

  description: [Pubmed|22383849]

Biological materials

Mutant

• MGNA-B485 (yhdR::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1484 NBRP B. subtilis, Japan]
• BKE09570 ([gene|8F7432F7EF0F767C6000D553A3ACE29FBA2EDCEE|yhdR]::erm  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE09570 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CATCTGTCCCTTCCGCATAT,  downstream forward: _UP4_TAAGAAGGCAAAGAAAAGAA
• BKK09570 ([gene|8F7432F7EF0F767C6000D553A3ACE29FBA2EDCEE|yhdR]::kan  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK09570 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CATCTGTCCCTTCCGCATAT,  downstream forward: _UP4_TAAGAAGGCAAAGAAAAGAA