yhcF

168

similar to transcriptional regulator ([wiki|GntR family])

locus: BSU_09060

Molecular weight: 13.81 kDa

pI: 5.46

Protein length: 121 aa

Gene length: 366 bp

function: unknown

product: transcriptional regulator ([wiki|GntR family])

essential: no

synonyms: yhcF

List of homologs in different organisms, belongs to COG1725 (Galperin et al., 2021)

Gene

Coordinates: 981,237  981,602

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The protein

Protein family

• [wiki|GntR family] of transcription factors

[wiki|Domains]

• [wiki|HTH gntR-type domain] (aa 9-77) (according to UniProt)

Structure

• [PDB|3NEU] (similar protein from ''Listeria innocua'', 38% identity)
• [AF|P54590]

Expression and Regulation

Operons

• genes: [gene|74B0D594308D313A54C7163759628655382B1F94|yhcE]-[gene|53E29A238EC817CE9B430BDED92DE8E9746C928B|yhcF]-[gene|E71E305A0DE5AB6BE24CE47DC1ECCD99670C24D8|yhcG]-[gene|70858AED096A80C1EAEB81F4B048368B665AB515|yhcH]-[gene|BAD93C5EAD25F1A257907F4C631C02FE97C7BC89|yhcI]

  description: [pubmed|22383849]

Biological materials

Mutant

• MGNA-B473 (yhcF::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1472 NBRP B. subtilis, Japan]
• BKE09060 ([gene|53E29A238EC817CE9B430BDED92DE8E9746C928B|yhcF]::erm  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE09060 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_GGATTGGAATTGATTGTCCA,  downstream forward: _UP4_AAAACATTCACAGAGGGAGG
• BKK09060 ([gene|53E29A238EC817CE9B430BDED92DE8E9746C928B|yhcF]::kan  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK09060 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_GGATTGGAATTGATTGTCCA,  downstream forward: _UP4_AAAACATTCACAGAGGGAGG

References

• 21815947,23504016