yhaX

168

[protein|3CB24D493B3282278B7DC61CE3C793DFA815F9E2|sigE]-dependent sporulation protein

locus: BSU_09830

Molecular weight: 32.26 kDa

pI: 8.07

Protein length: 288 aa

Gene length: 867 bp

function: protection of the spore

product: spore coat protein

essential: no

synonyms: yhaX

List of homologs in different organisms, belongs to COG0561 (Galperin et al., 2021)

Gene

Coordinates: 1,056,702 → 1,057,568

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The protein

Protein family

• [wiki|HAD superfamily] (according to UniProt)

Structure

• [PDB|3DNP]
• [AF|O07539]

[wiki|Localization]

• spore coat (basement), localization depends on [protein|A25C1530DA7BB007A288E525404E9F775E219FE8|spoIVA] [Pubmed|22171814]

Expression and Regulation

Operons

• genes: [gene|D7DD685750AB2C3CCDE0A2EBBEE1C1B9F97F2BB7|yhaX]

  description: [Pubmed|10498703]

  regulation

  • expressed during sporulation in the mother cell ([protein|search|SigE], [wiki|SpoIIID]) [Pubmed|15699190,12662922,15383836]

  regulatory mechanism

  • [protein|90DCE4F286D2C151D8BDBF0E024E6BBEC94E2397|spoIIID]: repression, [Pubmed|15383836], in [regulon|protein:90DCE4F286D2C151D8BDBF0E024E6BBEC94E2397|spoIIID regulon]

  sigma factors

  • [protein|3CB24D493B3282278B7DC61CE3C793DFA815F9E2|sigE]: sigma factor, [Pubmed|15699190,12662922], in [regulon|protein:3CB24D493B3282278B7DC61CE3C793DFA815F9E2|sigE regulon]

  additional information

  • A [protein|search|ncRNA] is predicted between '[protein|search|yhaZ]' and '[protein|search|yhaX]' [PubMed|20525796]

Biological materials

Mutant

• MGNA-A676 (yhaX::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/676 NBRP B. subtilis, Japan]
• BKE09830 (Δ[gene|D7DD685750AB2C3CCDE0A2EBBEE1C1B9F97F2BB7|yhaX]::erm  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE09830 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CACAGAGATGTCCCCCTCCT,  downstream forward: _UP4_TAAAAAAGCCGCTCGCGCCC
• BKK09830 (Δ[gene|D7DD685750AB2C3CCDE0A2EBBEE1C1B9F97F2BB7|yhaX]::kan  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK09830 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CACAGAGATGTCCCCCTCCT,  downstream forward: _UP4_TAAAAAAGCCGCTCGCGCCC

References

Reviews

• 23202530,27227299,35638784

Original Publications

• 10498703,15699190,12662922,20525796,22171814,15383836,33573199