yhaH

168

unknown

locus: BSU_10000

Molecular weight: 12.93 kDa

pI: 8.6

Protein length: 118 aa

Gene length: 357 bp

function: unknown

product: unknown

essential: no

synonyms: yhaH

List of homologs in different organisms, belongs to COG4980 (Galperin et al., 2021)

Gene

Coordinates: 1,073,895  1,074,251

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The protein

Structure

• [AF|O07516]

[wiki|Localization]

• cell membrane (according to UniProt)

Expression and Regulation

Operons

• genes: [gene|6EF9B43AFDE3AEBE0DA5EDCCEDA99331BA01AE5D|yhaH]

  description: [Pubmed|22383849]

Biological materials

Mutant

• GP3768 (Δ[gene|6EF9B43AFDE3AEBE0DA5EDCCEDA99331BA01AE5D|yhaH]::kan) available in [wiki|Jörg Stülke]'s lab
• GP3770 (Δ[gene|6EF9B43AFDE3AEBE0DA5EDCCEDA99331BA01AE5D|yhaH]::kan Δ[gene|43BF293F735ED783D36F33AA4701D57F2AED6657|ytxH]::cat) available in [wiki|Jörg Stülke]'s lab
• MGNA-A667 (yhaH::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/667 NBRP B. subtilis, Japan]
• BKE10000 ([gene|6EF9B43AFDE3AEBE0DA5EDCCEDA99331BA01AE5D|yhaH]::erm  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE10000 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CATTACAATCACCCCGTCTT,  downstream forward: _UP4_TAATATCCTATTCAAAAGAA
• BKK10000 ([gene|6EF9B43AFDE3AEBE0DA5EDCCEDA99331BA01AE5D|yhaH]::kan  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK10000 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CATTACAATCACCCCGTCTT,  downstream forward: _UP4_TAATATCCTATTCAAAAGAA

Expression vectors

• pGP3641: expression of [gene|6EF9B43AFDE3AEBE0DA5EDCCEDA99331BA01AE5D|yhaH] by [wiki|pBQ200] in B. subtilis, available in [wiki|Jörg Stülke]'s lab
• pGP3648: expression of Strep-[gene|6EF9B43AFDE3AEBE0DA5EDCCEDA99331BA01AE5D|yhaH] by [wiki|pGP380] in B. subtilis suitable for [wiki|SPINE], available in [wiki|Jörg Stülke]'s lab
• pGP3649: expression of [gene|6EF9B43AFDE3AEBE0DA5EDCCEDA99331BA01AE5D|yhaH]-Strep by [wiki|pGP382] in B. subtilis suitable for [wiki|SPINE], available in [wiki|Jörg Stülke]'s lab
• pGP3654 (N-terminal Strep-tag, purification from E. coli, in [wiki|pGP172]), available in [wiki|Jörg Stülke]'s lab
• pGP3670 (N-terminal His-tag, purification from E. coli, in [wiki|pWH844]), available in [wiki|Jörg Stülke]'s lab

two-hybrid system

• B. pertussis adenylate cyclase-based bacterial two hybrid system ([wiki|BACTH]), available in [wiki|Jörg Stülke]'s lab

References

Reviews

• 36882621

Original Publications

• 36815589