yfmJ

168

similar to predicted oxidoreductase, Zn-dependent and NAD(P)-binding

locus: BSU_07450

Molecular weight: 36.51 kDa

pI: 4.75

Protein length: 339 aa

Gene length: 1020 bp

function: unknown

product: unknown

essential: no

synonyms: yfmJ

List of homologs in different organisms, belongs to COG2130 (Galperin et al., 2021)

Gene

Coordinates: 817,810 → 818,829

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The protein

Protein family

• NADP-dependent oxidoreductase L4BD family (single member, according to UniProt)

Structure

• [PDB|4B7C] (from ''Pseudomonas Aeruginosa'', 48% identity)
• [AF|O34812]

Expression and Regulation

Operons

• genes: [gene|3DFC1A118B346C7939851BF711FFA31E04A698E8|yfmI]-[gene|B61458337D96A0CF8D0778A03A0DB68B566913D8|yfmJ]

  description: [Pubmed|22383849]

  regulation

  • expressed under conditions that trigger sporulation ([wiki|Spo0A]) [Pubmed|14651647]

  regulatory mechanism

  • [protein|2C54FE2ADC82FF414D732018C90649D477A925AD|spo0A]: activation, [Pubmed|14651647], in [regulon|protein:2C54FE2ADC82FF414D732018C90649D477A925AD|spo0A regulon]
  • [protein|E5110D9C36E8C55AFD1419987B14A53232165F20|abrB]: repression, [Pubmed|20817675], in [regulon|protein:E5110D9C36E8C55AFD1419987B14A53232165F20|abrB regulon]

  additional information

  • A [protein|search|ncRNA] is predicted between '[protein|3DFC1A118B346C7939851BF711FFA31E04A698E8|yfmI]' and '[protein|893BB8D1B43F10B46BB7F2DBFE9BE63E9AEEE8EB|yfmG]' [PubMed|20525796]

• genes: [gene|B61458337D96A0CF8D0778A03A0DB68B566913D8|yfmJ]

  description: [Pubmed|22383849]

Biological materials

Mutant

• MGNA-C241 (yfmJ::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/2239 NBRP B. subtilis, Japan]
• BKE07450 (Δ[gene|B61458337D96A0CF8D0778A03A0DB68B566913D8|yfmJ]::erm  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE07450 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CATTTTCATCCTCCTATCAC,  downstream forward: _UP4_TGAATGAAGATGAAAACGGG
• BKK07450 (Δ[gene|B61458337D96A0CF8D0778A03A0DB68B566913D8|yfmJ]::kan  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK07450 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CATTTTCATCCTCCTATCAC,  downstream forward: _UP4_TGAATGAAGATGAAAACGGG

References

• 14651647