fecE

168

iron/citrate ABC transporter (permease)

Locus: BSU_07500

Molecular weight: 35.06 kDa

Isoelectric point: 10.06

Protein length: 333 aa

Gene length: 1002 bp

Function: iron uptake

Product: iron/citrate ABC transporter (permease)

Essential: no

Synonyms: fecE, yfmE

Genomic Context

Hide small RNAsZoom:

List of homologs in different organisms, belongs to COG0609 (Galperin et al., 2021)

Gene

Coordinates: 823,716  824,717

The protein

Protein family

• Binding-protein-dependent transport system permease family (according to UniProt)
• FecCD subfamily (according to UniProt)

Structure

• 4G1U (PDB) (from Yersinia pestis, the FecD-FecE-FecF complex, 37% identity) PubMed
• 4R9U (PDB), the E. coli BtuC-BtuD complex, BtuC shares 35% identity, 66% similarity with YfmE, PubMed
• O34832 (AlphaFold)

Paralogous protein(s)

• FecD, FhuG, YfhA

Localization

• cell membrane PubMed

Expression and Regulation

Operons

• Genes: fecC-fecD-fecE-fecF

  Description: PubMed

  Regulation

  • immediately induced upon iron starvation (first wave to allow iron uptake) (Fur) PubMed

  Regulatory mechanism

  • Fur: repression, PubMed, in fur regulon

Biological materials

Mutant

• MGNA-C245 (yfmE::erm), available at the NBRP B. subtilis, Japan
• BKE07500 (fecE::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_TTTTTGTTTTCTGGTTGTTT,  downstream forward: _UP4_AGGAAACAGTAGGGAGAGGA
• BKK07500 (fecE::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_TTTTTGTTTTCTGGTTGTTT,  downstream forward: _UP4_AGGAAACAGTAGGGAGAGGA

References

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