yflN

168

similar to metallo-beta-lactamase

Locus: BSU_07620

Molecular weight: 28.68 kDa

Isoelectric point: 5.07

Protein length: 264 aa

Gene length: 795 bp

Function: unknown

Product: unknown

Essential: no

Synonyms: yflN

Genomic Context

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Categories containing this gene/protein

Lifestyles, Sporulation, Sporulation proteins, Newly identified sporulation proteins (based on transcription profiling)

List of homologs in different organisms, belongs to COG0491 (Galperin et al., 2021)

This gene is a member of the following regulons

SigA regulon, CcpA regulon, CitT regulon

Gene

Coordinates: 835,740  836,534

The protein

Protein family

metallo-beta-lactamase superfamily (according to UniProt)

Structure

4AWY (PDB) (from Pseudomonas aeruginosa, 25% identity) PubMed
O34409 (AlphaFold)

Expression and Regulation

Operons

Genes: citM-yflN

Description: PubMed

Regulation

induced by citrate (CitT) PubMed

Regulatory mechanism

CitT: activation, PubMed, in citT regulon
CcpA: repression, PubMed, in ccpA regulon

Sigma factors

SigA: sigma factor, PubMed, in sigA regulon

Biological materials

Mutant

MGNA-C253 (yflN::erm), available at the NBRP B. subtilis, Japan
BKE07620 (yflN::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATAATGACACCTCCGGAAT,  downstream forward: _UP4_TGACACCCGCACCACGCGGG
BKK07620 (yflN::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATAATGACACCTCCGGAAT,  downstream forward: _UP4_TGACACCCGCACCACGCGGG

References

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