yfjP

168

similar to DNA-3-methyladenine glycosidase II

locus: BSU_08010

Molecular weight: 33.03 kDa

pI: 5.52

Protein length: 287 aa

Gene length: 864 bp

function: unknown

product: unknown

essential: no

ec: 3.2.2.21

synonyms: yfjP

List of homologs in different organisms, belongs to COG0122 (Galperin et al., 2021)

Gene

Coordinates: 872,425  873,288

Advertisement

The protein

Catalyzed reaction/ biological activity

• Hydrolysis of alkylated DNA, releasing 3-methyladenine, 3-methylguanine, 7-methylguanine and 7-methyladenine (according to UniProt)

Protein family

• alkylbase DNA glycosidase AlkA family (with [protein|297DF91EF3E91A6D2DC39BBC7FE8711AB7EBE509|alkA], according to UniProt)

Structure

• [PDB|2H56] (from B. halodurans, corresponds to aa 119 ... 285, 29% identity)
• [AF|O31544]

Expression and Regulation

Operons

• genes: [gene|82CDC97D16426016CA7E75240C1A6D0D600F4375|yfjP]-[gene|05392BB2C32B0405799D4F34E5BA55FC936AB943|yfjO]

  description: [pubmed|22383849]

Biological materials

Mutant

• MGNA-C275 (yfjP::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/2273 NBRP B. subtilis, Japan]
• BKE08010 ([gene|82CDC97D16426016CA7E75240C1A6D0D600F4375|yfjP]::erm  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE08010 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CATGAGATCCACCCTTTTTT,  downstream forward: _UP4_TAATTCGTCTGACAATGAAG
• BKK08010 ([gene|82CDC97D16426016CA7E75240C1A6D0D600F4375|yfjP]::kan  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK08010 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CATGAGATCCACCCTTTTTT,  downstream forward: _UP4_TAATTCGTCTGACAATGAAG

References

Reviews

• 22933559