yfjO

168

similar to ribosomal RNA methyltransferase

locus: BSU_08020

Molecular weight: 51.97 kDa

pI: 9.81

Protein length: 466 aa

Gene length: 1401 bp

function: unknown

product: putative rRNA methyltransferase

essential: no

synonyms: yfjO

List of homologs in different organisms, belongs to COG2265 (Galperin et al., 2021)

Gene

Coordinates: 873,402  874,802

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The protein

Protein family

• [wiki|Methyltransferase superfamily] (according to UniProt)
• [wiki|class I-like SAM-binding methyltransferase superfamily] (according to UniProt)

[wiki|Domains]

• [wiki|TRAM domain] (aa 11-69) (according to UniProt)

[wiki|Cofactors]

• Fe-S cluster [pubmed|29292548]

Structure

• [PDB|5XJ1] (from Streptococcus pneumoniae, 35% identity) [pubmed|28949991]
• [AF|O31545]

Paralogous protein(s)

• [protein|B97ABE3233A27A7C8273ED9A1E688247DD37FD12|rlmCD]

Expression and Regulation

Operons

• genes: [gene|82CDC97D16426016CA7E75240C1A6D0D600F4375|yfjP]-[gene|05392BB2C32B0405799D4F34E5BA55FC936AB943|yfjO]

  description: [pubmed|22383849]

Biological materials

Mutant

• MGNA-C276 (yfjO::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/2274 NBRP B. subtilis, Japan]
• BKE08020 ([gene|05392BB2C32B0405799D4F34E5BA55FC936AB943|yfjO]::erm  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE08020 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CACGTTGTTCTCTCCGTTTC,  downstream forward: _UP4_TAAAAAGAACATTTCCCAGT
• BKK08020 ([gene|05392BB2C32B0405799D4F34E5BA55FC936AB943|yfjO]::kan  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK08020 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CACGTTGTTCTCTCCGTTTC,  downstream forward: _UP4_TAAAAAGAACATTTCCCAGT

References

• 21824914,28949991