yfiZ

168

ABC transporter for the siderophore schizokinen and arthrobactin (permease), works with ATPase YusV

Locus: BSU_08450

Molecular weight: 34.98 kDa

Isoelectric point: 9.62

Protein length: 333 aa

Gene length: 1002 bp

Function: acquisition of iron

Product: ABC transporter for the siderophore schizokinen and arthrobactin (permease)

Essential: no

Synonyms: yfiZ

Genomic Context

Hide small RNAsZoom:

List of homologs in different organisms, belongs to COG0609 (Galperin et al., 2021)

Gene

Coordinates: 920,474  921,475

The protein

Protein family

• Binding-protein-dependent transport system permease family (according to UniProt)
• FecCD subfamily (according to UniProt)

Structure

• 2NQ2 (PDB) (complex with YusV-like protein, from Haemophilus influenzae, 33% identity) PubMed
• O31568 (AlphaFold)

Paralogous protein(s)

• FecD, FhuB

Localization

• cell membrane PubMed

Expression and Regulation

Operons

• Genes: yfiZ-yfhA

  Description: PubMed

  Regulatory mechanism

  • Fur: repression, PubMed, in fur regulon

Biological materials

Mutant

• MGNA-C312 (yfiZ::erm), available at the NBRP B. subtilis, Japan
• BKE08450 (yfiZ::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATGATCTAATTCTCCTAGT,  downstream forward: _UP4_CTATGAAACTTCGTTTTGGC
• BKK08450 (yfiZ::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATGATCTAATTCTCCTAGT,  downstream forward: _UP4_CTATGAAACTTCGTTTTGGC

References

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