yfiZ

168

[wiki|ABC transporter] for the siderophore schizokinen and arthrobactin (permease), works with ATPase [protein|5C52DB31F378E49AABE5D937DD901A68F3810477|yusV]

locus: BSU_08450

Molecular weight: 34.98 kDa

pI: 9.62

Protein length: 333 aa

Gene length: 1002 bp

function: [wiki|acquisition of iron]

product: [wiki|ABC transporter] for the siderophore schizokinen and arthrobactin (permease)

essential: no

synonyms: yfiZ

List of homologs in different organisms, belongs to COG0609 (Galperin et al., 2021)

Gene

Coordinates: 920,474  921,475

The protein

Protein family

• [wiki|Binding-protein-dependent transport system permease family] (according to UniProt)
• [wiki|FecCD subfamily] (according to UniProt)

Structure

• [PDB|2NQ2] (complex with [protein|5C52DB31F378E49AABE5D937DD901A68F3810477|yusV]-like protein, from Haemophilus influenzae, 33% identity) [pubmed|17158291]
• [AF|O31568]

Paralogous protein(s)

• [protein|BDAA56484E05DA3C1CB0CA09873A365657999679|fecD], [protein|3E1C925C31C00803982C8EB1C6B025C22DB4A828|fhuB]

[wiki|Localization]

• cell membrane [Pubmed|10092453]

Expression and Regulation

Operons

• genes: [gene|81494D4E7E52531802C3C0D0073739DA9375F5DF|yfiZ]-[gene|34A7E22B4EF118EF5A76F8CC2DABBB023BC9A2E9|yfhA]

  description: [pubmed|22383849]

  regulatory mechanism

  • [protein|F899F1EE27E6D503BCC06BC52E3C7FD80B8EF725|fur]: repression, [pubmed|12354229], in [regulon|protein:F899F1EE27E6D503BCC06BC52E3C7FD80B8EF725|fur regulon]

Biological materials

Mutant

• MGNA-C312 (yfiZ::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/2310 NBRP B. subtilis, Japan]
• BKE08450 ([gene|81494D4E7E52531802C3C0D0073739DA9375F5DF|yfiZ]::erm  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE08450 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CATGATCTAATTCTCCTAGT,  downstream forward: _UP4_CTATGAAACTTCGTTTTGGC
• BKK08450 ([gene|81494D4E7E52531802C3C0D0073739DA9375F5DF|yfiZ]::kan  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK08450 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CATGATCTAATTCTCCTAGT,  downstream forward: _UP4_CTATGAAACTTCGTTTTGGC

References

• 36815589,10092453,16672620,12354229,17158291,39397791