yfiF

168

similar to transcription factor ([wiki|AraC family])

locus: BSU_08250

Molecular weight: 36.04 kDa

pI: 8.08

Protein length: 314 aa

Gene length: 945 bp

function: unknown

product: unknown

essential: no

synonyms: yfiF

List of homologs in different organisms, belongs to COG4977 (Galperin et al., 2021)

Gene

Coordinates: 898,961 → 899,905

The protein

Protein family

• [wiki|AraC family]

[wiki|Domains]

• [wiki|HTH araC/xylS-type domain] (aa 192-289) (according to UniProt)

Structure

• [PDB|1BL0] (from E. coli, the [wiki|HTH araC/xylS-type domain], 35% identity) [pubmed|9724717]
• [AF|P54722]

Expression and Regulation

Operons

• genes: [gene|CC5ED09FC661476160443898A083FF41A1F70DCE|yfiF]

  description: (according to [http://dbtbs.hgc.jp/COG/prom/yfiF.html DBTBS]) null

Biological materials

Mutant

• MGNA-C296 (yfiF::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/2294 NBRP B. subtilis, Japan]
• BKE08250 (Δ[gene|CC5ED09FC661476160443898A083FF41A1F70DCE|yfiF]::erm  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE08250 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CATGTAAACGCCTCCTAACA,  downstream forward: _UP4_TAAGATGTCCTGAAATGACA
• BKK08250 (Δ[gene|CC5ED09FC661476160443898A083FF41A1F70DCE|yfiF]::kan  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK08250 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CATGTAAACGCCTCCTAACA,  downstream forward: _UP4_TAAGATGTCCTGAAATGACA

References

• 23504016,9724717