yfiB

168

similar to ABC transporter (ATP-binding protein)

Locus: BSU_08210

Molecular weight: 63.77 kDa

Isoelectric point: 8.93

Protein length: 573 aa

Gene length: 1722 bp

Function: unknown

Product: ABC transporter (ATP-binding protein)

Essential: no

Synonyms: yfiB

Genomic Context

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Categories containing this gene/protein

Cellular processes, Transporters, ABC transporters, Exporters, Exporters of unknown function

Groups of genes, Membrane proteins

List of homologs in different organisms, belongs to COG1132 (Galperin et al., 2021)

Gene

Coordinates: 893,904  895,625

The protein

Protein family

ABC transporter superfamily (according to UniProt)

Domains

has both a membrane-spanning and an ATP-binding domain PubMed
ABC transmembrane type-1 domain (aa 15-298) (according to UniProt)
ABC transporter domain (aa 330-563) (according to UniProt)

Structure

3QF4 (PDB) (YfiB-YfiC complex from Thermotoga maritima, 44% identity) PubMed
P54718 (AlphaFold)

Paralogous protein(s)

BmrC, YknU, YknV, YwjA, YfiC, BmrA, YgaD

Localization

cell membrane PubMed

Expression and Regulation

Operons

Genes: yfiB-yfiC

Description: PubMed

Biological materials

Mutant

MGNA-C292 (yfiB::erm), available at the NBRP B. subtilis, Japan
BKE08210 (yfiB::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATGTAAAAGCCTCCAAACC,  downstream forward: _UP4_TTCGGAAGGGAGGGGAGCGA
BKK08210 (yfiB::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATGTAAAAGCCTCCAAACC,  downstream forward: _UP4_TTCGGAAGGGAGGGGAGCGA

References

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