yfhO

168

required for lipoteichoic acid glycosylation

locus: BSU_08610

Molecular weight: 93.56 kDa

pI: 9.63

Protein length: 861 aa

Gene length: 2586 bp

function: lipoteichoic acid glycosylation

product: glycosyltransferase

essential: no

synonyms: yfhO

List of homologs in different organisms, belongs to COG4485 (Galperin et al., 2021)

Gene

Coordinates: 931,879 → 934,464

Phenotypes of a mutant

• expression of [protein|824E6AE2FFA3CBC9F90648F15178A00A6CC49E4C|csbB] in the absence of [protein|BDFB6CCB5AAD9747971DE6B78E07558706E04617|yfhO] causes constitutive activation of [protein|081DF3EE9FA56209D648C7677188C61CE3AA8E41|sigM] [Pubmed|23632331]

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The protein

Catalyzed reaction/ biological activity

• moves GlcNAc sugar moieties from a C55-P intermediate onto the lipoteichoic acid backbone on the outside of the cell [pubmed|29343515]

[wiki|Domains]

• 13 predicted transmembrane helices and a large extracellular loop of 389 amino acids connecting the last two transmembrane helices [pubmed|29343515]

Structure

• [AF|O31582]

[wiki|Localization]

• cell membrane, with large extracellular loop [pubmed|29343515]

Expression and Regulation

Operons

• genes: [gene|824E6AE2FFA3CBC9F90648F15178A00A6CC49E4C|csbB]-[gene|BDFB6CCB5AAD9747971DE6B78E07558706E04617|yfhO]

  description: [Pubmed|22383849,8921856]

  regulation

  • induced by stress ([protein|580011DE5DC40EC9E7E0512791D328FAA010DCB8|sigB]) [Pubmed|8921856,15805528,9636707]

  sigma factors

  • [protein|580011DE5DC40EC9E7E0512791D328FAA010DCB8|sigB]: sigma factor, [Pubmed|8921856,9636707], in [regulon|protein:580011DE5DC40EC9E7E0512791D328FAA010DCB8|sigB regulon]
  • [protein|E77364F274A520FCCA9F5C9504E317B047BA29E6|sigX]: sigma factor, [Pubmed|9636707], in [regulon|protein:E77364F274A520FCCA9F5C9504E317B047BA29E6|sigX regulon]

Biological materials

Mutant

• MGNA-C320 (yfhO::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/2318 NBRP B. subtilis, Japan]
• BKE08610 (Δ[gene|BDFB6CCB5AAD9747971DE6B78E07558706E04617|yfhO]::erm  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE08610 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CATATGAATTCTCCAGTCTT,  downstream forward: _UP4_CTCGTCAGTCTGCTGCTTGC
• BKK08610 (Δ[gene|BDFB6CCB5AAD9747971DE6B78E07558706E04617|yfhO]::kan  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK08610 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CATATGAATTCTCCAGTCTT,  downstream forward: _UP4_CTCGTCAGTCTGCTGCTTGC

References

• 23632331,29343515