yesY

168

similar to rhamnogalacturonan acetylesterase

Locus: BSU_07070

Molecular weight: 24.45 kDa

Isoelectric point: 5.6

Protein length: 217 aa

Gene length: 654 bp

Function: unknown

Product: unknown

Essential: no

Synonyms: yesY

Genomic Context

Hide small RNAsZoom:

List of homologs in different organisms, belongs to COG2755 (Galperin et al., 2021)

Gene

Coordinates: 774,138  774,791

The protein

Protein family

• 'GDSL' lipolytic enzyme family (according to UniProt)

Structure

• 2O14 (PDB) (YxiM, 35% identity)
• O31528 (AlphaFold)

Paralogous protein(s)

• YesT, YxiM

Expression and Regulation

Operons

• Genes: yesW-yesX-yesY-yesZ

  Description: PubMed

  Regulation

  • induced by pectin PubMed
  • repressed by glucose (CcpA) PubMed

  Regulatory mechanism

  • CcpA: repression, PubMed, in ccpA regulon

• Genes: rhiL-rhiF-rhiG-rhiN-rhgR-yesT-yesU-yesV-yesW-yesX-yesY-yesZ

  Description: PubMed

  Regulation

  • induced by pectin (RhgR, RhgL) PubMed
  • repressed by glucose (CcpA) PubMed

  Regulatory mechanism

  • RhgR: activation, PubMed, in rhgR regulon
  • SigA: Sigma factor, PubMed, in sigA regulon
  • RhgL: activation, PubMed, in rhgL regulon
  • CcpA: repression, PubMed, in ccpA regulon

Biological materials

Mutant

• MGNA-B454 (yesY::erm), available at the NBRP B. subtilis, Japan
• BKE07070 (yesY::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATTTTTTCATTCCTCCTCG,  downstream forward: _UP4_ATCATCAAAGAAAGGTGAAA
• BKK07070 (yesY::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATTTTTTCATTCCTCCTCG,  downstream forward: _UP4_ATCATCAAAGAAAGGTGAAA

References

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