yeeK

168

inner spore coat protein

Locus: BSU_06850

Molecular weight: 15.78 kDa

Isoelectric point: 6.85

Protein length: 145 aa

Gene length: 438 bp

Function: protection of the spore

Product: spore coat protein

Essential: no

E.C.: null

Synonyms: yeeK

Genomic Context

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List of homologs in different organisms

Gene

Coordinates: 753,265 → 753,702

The protein

Structure

• O31510 (AlphaFold)

Localization

• inner spore coat  PubMed,  assembles into the spore integument PubMed
• localization depends on CotE  PubMed

Additional information

• CotE, SafA, and SpoVID are required for proper localization, clolocalizes with YabG PubMed

Expression and Regulation

Operons

• Genes: yeeK

  Description: PubMed

  Regulation

  • expression starts 5 hrs after the onset of sporulation (SigK, GerE) PubMed

  Regulatory mechanism

  • GerE: activation, PubMed, in gerE regulon

  Sigma factors

  • SigK: sigma factor, PubMed, in sigK regulon

Biological materials

Mutant

• MGNA-A953 (yeeK::erm), available at the NBRP B. subtilis, Japan
• 1S125 ( yeeK::erm), PubMed, available at BGSC
• BKE06850 (ΔyeeK::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATATTTACTCTCTCCTTCA,  downstream forward: _UP4_TAAAGAAGATGATGATAAAA
• BKK06850 (ΔyeeK::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATATTTACTCTCTCCTTCA,  downstream forward: _UP4_TAAAGAAGATGATGATAAAA

References

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