yeeF

168

polymorphic LXG toxin, non-specific metal-dependent DNase

Locus: BSU_06812

Molecular weight: 74.00 kDa

Isoelectric point: 8.942

Protein length: 669 aa

Gene length: 2010 bp

Function: competetion with other bacteria in biofilms

Product: LXG toxin, non-specific metal-dependent DNase

Essential: no

E.C.: null

Synonyms: yeeF

Genomic Context

Hide small RNAsZoom:

List of homologs in different organisms, belongs to COG5444 (Galperin et al., 2021)

Gene

Coordinates: 747,554  749,563

Phenotypes of a mutant

• the mutant is outcompeted by wild type cells in biofilms PubMed

The protein

Catalyzed reaction/ biological activity

• non-specific metal-dependent DNase activity PubMed

Domains

• LXG domain (aa 1-235) (according to UniProt)

Structure

• O31506 (AlphaFold)

Localization

• secreted
• secretion and delivery requires WXG100 and the T7SS (YukD-EssB-YukB-YueB-YueC-YueD) PubMed

Expression and Regulation

Operons

• Genes: yeeF-yezG

  Description: PubMed

Biological materials

Mutant

• MGNA-A963 (yeeF::erm), available at the NBRP B. subtilis, Japan
• BKE06812 (yeeF::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATGACCATTTCCCTTCTTT,  downstream forward: _UP4_TAGAAGAATGGAAACTAGGA
• BKK06812 (yeeF::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATGACCATTTCCCTTCTTT,  downstream forward: _UP4_TAGAAGAATGGAAACTAGGA

References

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