ydjI

168

required for membrane localization of PspA (together with YdjG and YdjH)

Locus: BSU_06210

Molecular weight: 35.92 kDa

Isoelectric point: 4.94

Protein length: 323 aa

Gene length: 972 bp

Function: targeting of PspA

Product: unknown

Essential: no

E.C.: null

Synonyms: ydjI

Genomic Context

Hide small RNAsZoom:

List of homologs in different organisms, belongs to COG4260 (Galperin et al., 2021)

Gene

Coordinates: 673,814 → 674,785

Phenotypes of a mutant

• reduced membrane fluidity PubMed

The protein

Catalyzed reaction/ biological activity

• membrane localization of PspA (together with YdjG and YdjH) PubMed

Protein family

• Stomatin, Prohibitin, Flotillin, and HflK/C (SPFH) domain protein family (with FloA and FloT)

Domains

• contains a SPFH domain (Stomatin, Prohibitin, Flotillin, and HflK/C-Domain) (aa 22 - 232) PubMed
• C-terminal zinc-finger domain (aa 300 - 322) PubMed

Structure

• O34789 (AlphaFold)

Localization

• cell membrane (fluid regions) (via YdjG and YdjH)) PubMed

Expression and Regulation

Operons

• Genes: pspA-ydjG-ydjH-ydjI

  Description: PubMed

  Regulation

  • induced by alkaline shock (SigW) PubMed

  Regulatory mechanism

  • AbrB: repression, PubMed, in abrB regulon

  Sigma factors

  • SigW: sigma factor, PubMed, in sigW regulon

Biological materials

Mutant

• MGNA-C216 (ydjI::erm), available at the NBRP B. subtilis, Japan
• BKE06210 (ΔydjI::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATTACGCCTCTCCCTTTCT,  downstream forward: _UP4_TAAGAATCATACAGTGAAAA
• BKK06210 (ΔydjI::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATTACGCCTCTCCCTTTCT,  downstream forward: _UP4_TAAGAATCATACAGTGAAAA

References

Loading