ydiS

ydiS
168

component of the BsuM DNA restriction system, similar to 5-methylcytosine-specific restriction enzyme

locus
BSU_06100
Molecular weight
39.88 kDa
pI
8.7
Protein length
Gene length
function
BsuM DNA restriction
product
unknown
essential
no
synonyms
ydiS

Genomic Context

Categories containing this gene/protein

List of homologs in different organisms, belongs to COG1401 (Galperin et al., 2021)

This gene is a member of the following regulons

Gene
Coordinates
660,596  661,627
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The protein
Catalyzed reaction/ biological activity
Endonucleolytic cleavage of DNA to give specific double-stranded fragments with terminal 5'-phosphates (according to UniProt)
Structure
[PDB|6HZ4] (5-methylcytosine-specific restriction enzyme from E. coli, corresponds to aa 29 ... 223, 29% identity) [pubmed|31296862]
[AF|O34885]
Expression and Regulation
Operons
genes
[gene|4BD4CFB10700907DF1BBF3C9CFACAF573588E85F|ydiR]-[gene|A8C606348C8C6FC3BB9786C06C9BFE9D3519A041|ydiS]-[gene|D36B91B3C8EAFEB80A7395C62A50A5F80B8CC5C5|ydjA]
description
[Pubmed|11751814]
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[gene|4BD4CFB10700907DF1BBF3C9CFACAF573588E85F|ydiR]→[gene|D36B91B3C8EAFEB80A7395C62A50A5F80B8CC5C5|ydjA]

2024-03-23 22:51:24

ghost

34

593080e74f00e0eaa9810979a0d8f9d56acf2063

D2E7F16746984FED7A4AC060DFE140245634100E

Biological materials
Mutant
MGNA-C208 (ydiS::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/2206 NBRP B. subtilis, Japan]
DELTA0 (''ydiS  trpC2''), available at BGSC
BKE06100 ([gene|A8C606348C8C6FC3BB9786C06C9BFE9D3519A041|ydiS]::erm  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE06100 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CATCAGTTCATTTCCCCTAT,  downstream forward: _UP4_TCGTCTTTTGTGAGTGATTA
BKK06100 ([gene|A8C606348C8C6FC3BB9786C06C9BFE9D3519A041|ydiS]::kan  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK06100 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CATCAGTTCATTTCCCCTAT,  downstream forward: _UP4_TCGTCTTTTGTGAGTGATTA
References
11751814,22092861,31296862

A8C606348C8C6FC3BB9786C06C9BFE9D3519A041

Page visits: 1907

Time of last update: 2024-03-26 16:41:35

Author of last update: Jstuelk