ydgE

168

protein N-acetyltransferase, may acetylate [protein|EFD8FC875B6787B1512C5FC4C81E7E4C9BD93028|HBsu]

locus: BSU_05600

Molecular weight: 18.23 kDa

pI: 5.09

Protein length: 157 aa

Gene length: 474 bp

function: unknown

product: unknown

essential: no

synonyms: ydgE

List of homologs in different organisms, belongs to COG0456 (Galperin et al., 2021)

Gene

Coordinates: 604,103  604,576

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The protein

Catalyzed reaction/ biological activity

• may acetylate [protein|EFD8FC875B6787B1512C5FC4C81E7E4C9BD93028|HBsu] on Lys-41 and Lys-86 [pubmed|30808761]

Protein family

• [wiki|Acetyltransferase family] (according to UniProt)

[wiki|Domains]

• [wiki|N-acetyltransferase domain] (aa 3-157) (according to UniProt)

Structure

• [PDB|1UFH] ([protein|A1B9CF581D2D73AC3D2E290E6B86AD63BE0544DE|yycN], 42% identity) [pubmed|14635137]
• [AF|P96703]

Modification

• can self-acetylate [pubmed|30808761]

Paralogous protein(s)

• [protein|A1B9CF581D2D73AC3D2E290E6B86AD63BE0544DE|yycN]

Expression and Regulation

Operons

• genes: [gene|0C2CD998B933D7458DBC4E80A596801274AC43F9|ydgC]-[gene|6DD46B2E342994CB97C13C00AB264B2D62184223|ydgD]-[gene|07A30814F57112196051289AA309AE6DB22CAEFF|ydgE]

  description: [Pubmed|22383849]

Biological materials

Mutant

• MGNA-C158 (ydgE::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/2156 NBRP B. subtilis, Japan]
• BKE05600 ([gene|07A30814F57112196051289AA309AE6DB22CAEFF|ydgE]::erm  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE05600 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CATCCTGTATTCCTCCTTTA,  downstream forward: _UP4_TGAAAAGCCGCTTACGTTAC
• BKK05600 ([gene|07A30814F57112196051289AA309AE6DB22CAEFF|ydgE]::kan  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK05600 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CATCCTGTATTCCTCCTTTA,  downstream forward: _UP4_TGAAAAGCCGCTTACGTTAC

References

Research papers

• 14635137,30808761