ydfS

168

required for wet heat resistance of spores

Locus: BSU_05540

Molecular weight: 26.96 kDa

Isoelectric point: 8.45

Protein length: 235 aa

Gene length: 708 bp

Function: stabilization of spore inner membrane proteins against wet heat

Product: unknown

Essential: no

Synonyms: ydfS

Genomic Context

Hide small RNAsZoom:

List of homologs in different organisms, belongs to COG2323 (Galperin et al., 2021)

Gene

Coordinates: 601,019 → 601,726

Phenotypes of a mutant

• reduced wet heat resistance PubMed
• deficient in spore germination in response to AGFK PubMed

The protein

Protein family

• UPF0702 family (according to UniProt)

Structure

• 3C6F (PDB) (YetF, corresponds to aa 81 ... 230, 36% identity)
• P96697 (AlphaFold)

Paralogous protein(s)

• YetF

Localization

• inner spore membrane PubMed

Expression and Regulation

Operons

• Genes: ydfS

  Description: PubMed

  Regulation

  • expressed during sporulation in the forespore (SigG) PubMed

  Sigma factors

  • SigG: sigma factor, PubMed, in sigG regulon

Biological materials

Mutant

• MGNA-C156 (ydfS::erm), available at the NBRP B. subtilis, Japan
• BKE05540 (ΔydfS::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATGTTATGGCGCTCCCCGC,  downstream forward: _UP4_AAGGAATGAAGGAACAATCA
• BKK05540 (ΔydfS::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATGTTATGGCGCTCCCCGC,  downstream forward: _UP4_AAGGAATGAAGGAACAATCA

References

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