conG

168

part of the ICEBs1 conjugation machinery, exclusion specificity factor

Locus: BSU_04960

Molecular weight: 90.42 kDa

Isoelectric point: 6.29

Protein length: 815 aa

Gene length: 2448 bp

Function: conjugative transfer of ICEBs1

Product: part of the ICEBs1 conjugation machinery

Essential: no

E.C.: null

Synonyms: conG, yddG

Genomic Context

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List of homologs in different organisms, belongs to COG3064 (Galperin et al., 2021)

Gene

Coordinates: 541,578  544,025

Phenotypes of a mutant

• resistant to exclusion of ICEBs1 PubMed

The protein

Structure

• P96644 (AlphaFold)

Localization

• cell membrane, integral membrane protein PubMed

Expression and Regulation

Operons

• Genes: xis-ydzL-devI-ydcP-ydcQ-nicK-S172-ramS-ramT-ramA-conB-conC-conD-conE-yddF-conG-cwlT-yddI-yddJ

  Description: PubMed

  Regulatory mechanism

  • ImmR: repression, PubMed, in immR regulon

Biological materials

Mutant

• BKE04960 (conG::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_TTTCTTTTTCATTACAACCA,  downstream forward: _UP4_CTGAGAAGGGATGAAAGAAC
• BKK04960 (conG::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_TTTCTTTTTCATTACAACCA,  downstream forward: _UP4_CTGAGAAGGGATGAAAGAAC

References

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