ydaD

168

general stress protein, similar to alcohol dehydrogenase

Locus: BSU_04190

Molecular weight: 30.93 kDa

Isoelectric point: 4.78

Protein length: 286 aa

Gene length: 861 bp

Function: unknown

Product: unknown

Essential: no

Synonyms: ydaD

Genomic Context

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List of homologs in different organisms, belongs to COG1028 (Galperin et al., 2021)

Gene

Coordinates: 471,709  472,569

The protein

Protein family

• Short-chain dehydrogenases/reductases (SDR) family (according to UniProt)

Structure

• 3I30 (PDB) (from Bacillus Anthracis, 61% identity)
• P80873 (AlphaFold)

Paralogous protein(s)

• YhdF, BacC
• FabG, (31.1%)
• YkvO, (37.5%),
• YhxC, (63,5%)
• YhxD, (48,8%)
• YcdF, (69%)
• Gdh, (35,8%)
• YjdA, (33,3%)
• KduD, (36.7%)
• BacC (34.3%), FabG (31.1%), KduD (36.7%), YhdF

Expression and Regulation

Operons

• Genes: ydaD-ydaE

  Description: PubMed

  Regulation

  • induced by stress (SigB) PubMed

  Sigma factors

  • SigB: sigma factor, PubMed, in sigB regulon

• Genes: ydaD-ydaE-ydaF-ydaG

  Description: PubMed

  Regulation

  • induced by stress (SigB) PubMed

  Expression

  • expression is increased upon depletion of depletion of tRNA maturation factors rnpB or rnz PubMed

  Sigma factors

  • SigB: sigma factor, PubMed, in sigB regulon

Biological materials

Mutant

• MGNA-C086 (ydaD::erm), available at the NBRP B. subtilis, Japan
• BKE04190 (ydaD::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATGATGATTCTCCTCCTTT,  downstream forward: _UP4_ACGACATAAGAGGGAGTGAG
• BKK04190 (ydaD::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATGATGATTCTCCTCCTTT,  downstream forward: _UP4_ACGACATAAGAGGGAGTGAG

References

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