ycnE

168

putative monooxygenase

locus: BSU_03870

Molecular weight: 10.81 kDa

pI: 4.87

Protein length: 95 aa

Gene length: 288 bp

function: unknown

product: unknown

essential: no

synonyms: ycnE

List of homologs in different organisms, belongs to COG1359 (Galperin et al., 2021)

Gene

Coordinates: 439,282 → 439,569

Advertisement

The protein

Protein family

• LsrG family (single member, according to UniProt)

[wiki|Domains]

• [wiki|ABM domain] (aa 2-93) (according to UniProt)

Structure

• [PDB|1X7V] (from Pseudomonas aeruginosa, 38% identity) [pubmed|16049913]
• [AF|P94425]

Modification

• phosphorylation on Ser-24 [Pubmed|17218307]

Expression and Regulation

Operons

• genes: [gene|C8B3C93479DC1EF6DAD6FCBEEBAD57817BBAFDDA|ycnE]-[gene|BA6C61C488659D5F1B5237BA817A8A5AC6D0E988|ycnD]

  description: [Pubmed|17407181]

  regulation

  • induced by chromanon [Pubmed|17407181]

Biological materials

Mutant

• BKE03870 (Δ[gene|C8B3C93479DC1EF6DAD6FCBEEBAD57817BBAFDDA|ycnE]::erm  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE03870 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CATGTAAATCTCCTCCGTCG,  downstream forward: _UP4_AGCGAGTAAAGGAAGGGTGT
• BKK03870 (Δ[gene|C8B3C93479DC1EF6DAD6FCBEEBAD57817BBAFDDA|ycnE]::kan  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK03870 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CATGTAAATCTCCTCCGTCG,  downstream forward: _UP4_AGCGAGTAAAGGAAGGGTGT

References

• 17407181,17218307,16049913