fpbQ

168

petrobactin (3.4-catecholate siderophore) [wiki|ABC transporter] (binding protein), major component of the secretome

locus: BSU_03830

Molecular weight: 34.64 kDa

pI: 6.63

Protein length: 317 aa

Gene length: 954 bp

function: [wiki|acquisition of iron]

product: petrobactin [wiki|ABC transporter]  (binding protein)

essential: no

synonyms: fpbQ, yclQ, fpiA

List of homologs in different organisms, belongs to COG4607 (Galperin et al., 2021)

Gene

Coordinates: 435,036 → 435,989

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The protein

Catalyzed reaction/ biological activity

• uptake of the siderophore petrobactin [Pubmed|19955416]

Protein family

• [wiki|Bacterial solute-binding protein 8 family] (according to UniProt)

[wiki|Domains]

• [wiki|Fe/B12 periplasmic-binding domain] (aa 56-317) (according to UniProt)

Structure

• [PDB|3GFV]
• [AF|P94421]

[wiki|Localization]

• associated to the membrane (via [protein|552D4C42DCEA4625000160D2625711C61AB11661|fpbN]-[protein|EC63B5CDFDB4DF3A967ECEC40F2C44849311148B|fpbO]) [Pubmed|10092453,18763711]
• extracellular (signal peptide) [Pubmed|18957862]

Expression and Regulation

Operons

• genes: [gene|552D4C42DCEA4625000160D2625711C61AB11661|fpbN]-[gene|EC63B5CDFDB4DF3A967ECEC40F2C44849311148B|fpbO]-[gene|473628F86C18FE9957841F3C8B45242C90CB341D|fpbP]-[gene|C1D6D188E5BD039F32427B2A58BBFC0CC173A476|fpbQ]

  description: [Pubmed|19955416]

  regulation

  • immediately induced upon iron starvation (first wave to allow iron uptake) ([protein|F899F1EE27E6D503BCC06BC52E3C7FD80B8EF725|fur]) [Pubmed|29133393,12354229]

  regulatory mechanism

  • [protein|F899F1EE27E6D503BCC06BC52E3C7FD80B8EF725|fur]: repression, [pubmed|12354229], in [regulon|protein:F899F1EE27E6D503BCC06BC52E3C7FD80B8EF725|fur regulon]

additional information

• the mRNA is cleaved by [protein|BAB297D18F94FFA67B8D12A684AB4D1BCDFB4981|RNase III] [PubMed|26883633]

Biological materials

Mutant

• MGNA-C067 (yclQ::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/2065 NBRP B. subtilis, Japan]
• BKE03830 (Δ[gene|C1D6D188E5BD039F32427B2A58BBFC0CC173A476|fpbQ]::erm  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE03830 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CATGCTCCTCACCTCTTCTA,  downstream forward: _UP4_TAAAACCAAAAAGAGCCTCC
• BKK03830 (Δ[gene|C1D6D188E5BD039F32427B2A58BBFC0CC173A476|fpbQ]::kan  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK03830 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CATGCTCCTCACCTCTTCTA,  downstream forward: _UP4_TAAAACCAAAAAGAGCCTCC

References

• 10092453,19955416,12354229,18957862,18763711,26883633,29133393