folEB

168

GTP cyclohydrolase IB, required for the initial step of queuosine synthesis ([wiki|tRNA modification]), replaces [protein|7732A37D59E2643F232F2C0AFE51BCF7A79EDE29|folE] under conditions of zinc starvation

locus: BSU_03340

Molecular weight: 34.63 kDa

pI: 7.83

Protein length: 305 aa

Gene length: 915 bp

function: biosynthesis of folate and queuosine

product: zinc-independent GTP cyclohydrolase IB

essential: no

ec: 3.5.4.16

synonyms: folEB, folE2, yciA

List of homologs in different organisms, belongs to COG1469 (Galperin et al., 2021)

Gene

Coordinates: 364,259 → 365,173

Advertisement

The protein

Catalyzed reaction/ biological activity

• GTP + H₂O --> 7,8-dihydroneopterin 3'-triphosphate + formate + H⁺ (according to UniProt)

Protein family

• GTP cyclohydrolase IV family (single member, according to UniProt)

[wiki|Cofactors]

• activated by a variety of divalent cations [Pubmed|19767425]

Structure

• [PDB|3D1T] [Pubmed|19767425]
• [AF|P94398]

Biological materials

Mutant

• BKE03340 (Δ[gene|93380E8EEB672C14C9BE2B5A6D8573FBCA149514|folEB]::erm  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE03340 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CATTCAGAAAACTCCTCTCA,  downstream forward: _UP4_TTTTCGAAGGAAGTGGATAA
• BKK03340 (Δ[gene|93380E8EEB672C14C9BE2B5A6D8573FBCA149514|folEB]::kan  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK03340 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CATTCAGAAAACTCCTCTCA,  downstream forward: _UP4_TTTTCGAAGGAAGTGGATAA

References

• 9811636,12426338,17032654,19767425,27561249