ycbM

168

two-component sensor kinase

locus: BSU_02560

Molecular weight: 17.85 kDa

pI: 9.75

Protein length: 311 aa

Gene length: 936 bp

function: unknown

product: two-component sensor kinase

essential: no

synonyms: ycbM

List of homologs in different organisms, belongs to COG2205 (Galperin et al., 2021)

Gene

Coordinates: 279,059  279,994

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The protein

Catalyzed reaction/ biological activity

• autophosphorylation, phosphorylation of [protein|60DA46B02BB5F71337AF5F34921E2E68EC062EC7|ycbL] (putative)
• ATP + protein L-histidine --> ADP + protein N-phospho-L-histidine

[wiki|Domains]

• [wiki|Histidine kinase domain] (aa 92-310) (according to UniProt)

Structure

• [PDB|5C93] ([protein|E88978809272FAC2520DB4ABCA0554F8028F3451|walK] from Lactobacillus plantarum, 27% identity) [pubmed|28994408]
• [AF|P42245]

Modification

• autophosphorylation on a His residue

[wiki|Localization]

• cell membrane (according to UniProt)

Biological materials

Mutant

• MGNA-C035 (ycbM::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/2033 NBRP B. subtilis, Japan]
• BKE02560 ([gene|348F81B6ECB6FFBEC092A672FF5F40FE86918872|ycbM]::erm  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE02560 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_AGCGGCCACCCATAGCACTG,  downstream forward: _UP4_TAATCGTAAGAATTTCTTAA
• BKK02560 ([gene|348F81B6ECB6FFBEC092A672FF5F40FE86918872|ycbM]::kan  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK02560 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_AGCGGCCACCCATAGCACTG,  downstream forward: _UP4_TAATCGTAAGAATTTCTTAA

References

• 10094672,28994408