ybxI

168

beta-lactamase

locus: BSU_02090

Molecular weight: 30.49 kDa

pI: 6.84

Protein length: 267 aa

Gene length: 804 bp

function: unknown

product: beta-lactamase

essential: no

ec: 3.5.2.6

synonyms: ybxI, ybdS

List of homologs in different organisms, belongs to COG2602 (Galperin et al., 2021)

Gene

Coordinates: 228,549 → 229,352

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The protein

Catalyzed reaction/ biological activity

• β-lactam + H₂O --> substituted β-amino acid (according to UniProt)

Protein family

• [wiki|beta-lactamase family] (according to UniProt)
• class-D beta-lactamase family (single member, according to UniProt)

Structure

• [PDB|5E2F]
• [AF|P54427]

[wiki|Localization]

• extracellular (signal peptide) [Pubmed|18957862]

Expression and Regulation

Operons

• genes: [gene|E1CBB22683967FA47CB5886755BC3C3BFE6CC499|ybxI]

  description: [Pubmed|22383849]

Biological materials

Mutant

• MGNA-B962 (ybxI::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1961 NBRP B. subtilis, Japan]
• BKE02090 (Δ[gene|E1CBB22683967FA47CB5886755BC3C3BFE6CC499|ybxI]::erm  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE02090 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CATTTTGTTCTCCCCTTCGT,  downstream forward: _UP4_TAAATAAAAGAGCCCTGCAC
• BKK02090 (Δ[gene|E1CBB22683967FA47CB5886755BC3C3BFE6CC499|ybxI]::kan  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK02090 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CATTTTGTTCTCCCCTTCGT,  downstream forward: _UP4_TAAATAAAAGAGCCCTGCAC

References

• 18957862,26551395