rplGB

168

highly expressed protein, similar to ribosomal protein L7AE family, associated with the ribosome during exponential growth, binds K-turns in RNA switches

locus: BSU_01090

Molecular weight: 8.33 kDa

pI: 9.51

Protein length: 82 aa

Gene length: 249 bp

function: unknown

product: unknown

essential: no

synonyms: ybxF, ybaB, rpmXB, ktuS

List of homologs in different organisms, belongs to COG1358 (Galperin et al., 2021)

Gene

Coordinates: 129,340  129,588

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The protein

Catalyzed reaction/ biological activity

• binds K-turns in [wiki|RNA switch]es as they occur in the [protein|search|L-box], the [protein|search|S-box] and the [protein|search|T-box] [Pubmed|22355167]

Protein family

• Eukaryotic ribosomal protein eL8 family (with [protein|7BFA65857F69855DBB3BB64D93B4ED2F09DC705B|rplGA], according to UniProt)

Structure

• [PDB|3V7E] ([protein|11D7ABA61FC4069B48614875AF2C6C694291B545|rplGB] bound to the [[S-box|SAM-I riboswitch]] aptamer) [Pubmed|22355167]
• [AF|P46350]

[wiki|Localization]

• cytoplasm (according to UniProt)

Biological materials

Mutant

• BKE01090 ([gene|11D7ABA61FC4069B48614875AF2C6C694291B545|rplGB]::erm  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE01090 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CATAAACATATCCTCCAAAG,  downstream forward: _UP4_TAACGTACTTTTGTTTTTGC
• BKK01090 ([gene|11D7ABA61FC4069B48614875AF2C6C694291B545|rplGB]::kan  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK01090 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CATAAACATATCCTCCAAAG,  downstream forward: _UP4_TAACGTACTTTTGTTTTTGC

References

• 11948165,23249744,22355167,26522935,29280348,29794222,17468242