yaaI

168

general stress protein, similar to isochorismatase

Locus: BSU_00170

Molecular weight: 20.66 kDa

Isoelectric point: 4.67

Protein length: 181 aa

Gene length: 546 bp

Function: survival of ethanol stress

Product: unknown

Essential: no

Synonyms: yaaI

Genomic Context

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Categories containing this gene/protein

Lifestyles, Coping with stress, General stress proteins (controlled by SigB)

Groups of genes, Poorly characterized/ putative enzymes

List of homologs in different organisms, belongs to COG1335 (Galperin et al., 2021)

This gene is a member of the following regulons

SigB regulon

Gene

Coordinates: 25,221  25,766

The protein

Protein family

Isochorismatase family (according to UniProt)

Structure

3TG2 (PDB) (from Vibrio cholerae, 26% identity) PubMed
P37532 (AlphaFold)

Additional information

important for survival of ethanol stress PubMed

Expression and Regulation

Operons

Genes: yaaI-yaaH

Description: PubMed

Regulation

induced by stress (SigB) PubMed

Sigma factors

SigB: sigma factor, PubMed, in sigB regulon

Biological materials

Mutant

MGNA-B890 (yaaI::erm), available at the NBRP B. subtilis, Japan
BKE00170 (yaaI::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CAATCGACAGTCTCCTTTCC,  downstream forward: _UP4_TAAACATGATCAGCGCTTTT
BKK00170 (yaaI::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CAATCGACAGTCTCCTTTCC,  downstream forward: _UP4_TAAACATGATCAGCGCTTTT

References

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