xylB

168

xylulokinase

locus: BSU_17610

Molecular weight: 55.25 kDa

pI: 6.46

Protein length: 499 aa

Gene length: 1500 bp

function: utilization of xylan and xylose

product: xylulokinase

essential: no

ec: 2.7.1.17

synonyms: xylB, yncA

List of homologs in different organisms, belongs to COG1070 (Galperin et al., 2021)

Gene

Coordinates: 1,893,396 → 1,894,895

The protein

Catalyzed reaction/ biological activity

• ATP + D-xylulose --> ADP + D-xylulose 5-phosphate + H⁺ (according to UniProt)

Protein family

• [wiki|FGGY kinase family] (according to UniProt)

Structure

• [PDB|2NLX] (from ''E. coli'', 35% identity, 52% similarity) [Pubmed|17123542]
• [AF|P39211]

Expression and Regulation

Operons

• genes: [gene|24829427B4518FE67038F194B54D4AAFAC64EF19|xylA]-[gene|F417A5B46CC7FC69DC8606A46D1AD5E41FA35B13|xylB]

  description: [Pubmed|1921970]

  regulation

  • carbon catabolite repression ([protein|search|CcpA]) [Pubmed|8132469]

  regulatory mechanism

  • [protein|E39AFEB7B7F28969A35C4B51CC6E7819F71C79D9|ccpA]: repression, [Pubmed|8132469], in [regulon|protein:E39AFEB7B7F28969A35C4B51CC6E7819F71C79D9|ccpA regulon]
  • [protein|AF4395D134485290F4AA307B48494FED39E52CD7|xylR]: repression, [Pubmed|2454911], in [regulon|protein:AF4395D134485290F4AA307B48494FED39E52CD7|xylR regulon]

  sigma factors

  • [protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA]: sigma factor, [Pubmed|2454911], in [regulon|protein:360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA regulon]

Biological materials

Mutant

• GP1151 (del aphA3) available in [wiki|Stülke] lab
• 1A719 (no resistance), [Pubmed|1719948], available at [http://pasture.asc.ohio-state.edu/BGSC/getdetail.cfm?bgscid=1A719&Search=1A719 BGSC]
• BKE17610 (Δ[gene|F417A5B46CC7FC69DC8606A46D1AD5E41FA35B13|xylB]::erm  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE17610 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CATTTTTTCACTCCTTGCCC,  downstream forward: _UP4_TAATGATGTTATTGTCTGGA
• BKK17610 (Δ[gene|F417A5B46CC7FC69DC8606A46D1AD5E41FA35B13|xylB]::kan  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK17610 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CATTTTTTCACTCCTTGCCC,  downstream forward: _UP4_TAATGATGTTATTGTCTGGA

labs

• [wiki|Wolfgang Hillen], Erlangen University, Germany [http://www.biologie.uni-erlangen.de/mibi/index2.html Homepage]

References

• 17123542,1921970,2454911,8132469