xlyB

168

N-acetylmuramoyl-L-alanine amidase

locus: BSU_12460

Molecular weight: 33.71 kDa

pI: 9.8

Protein length: 317 aa

Gene length: 954 bp

function: PBSX prophage-mediated lysis

product: N-acetylmuramoyl-L-alanine amidase

essential: no

ec: 3.5.1.28

synonyms: xlyB, yjpB

List of homologs in different organisms, belongs to COG5632 (Galperin et al., 2021)

Gene

Coordinates: 1,317,535  1,318,488

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The protein

Catalyzed reaction/ biological activity

• Hydrolyzes the link between N-acetylmuramoyl residues and L-amino acid residues in certain cell-wall glycopeptides (according to UniProt)

Protein family

• [wiki|N-acetylmuramoyl-L-alanine amidase 2 family] (according to UniProt)

[wiki|Domains]

• contains a N-acetylglucosamine-polymer-binding [wiki|LysM domain] [Pubmed|18430080]
• contains an amidase_2 domain (like [protein|C1EB8D05386C1B35B8002239F5247CC6AB25F786|blyA], [protein|C9C395A5F6A2C06EAC97F228C5C66D1628C937CF|cwlA], [protein|D5612882F1887BE342EF7072E71502382AB7289F|cwlH], [protein|A8AF827637B7208DFA101C9A2911BF9AB1AED08F|xlyB])
• [wiki|N-acetylmuramoyl-L-alanine amidase domain] (aa 40-142) (according to UniProt)
• [wiki|LysM domain] (aa 177-221) (according to UniProt)

Structure

• [PDB|2L47] (from Bacillus phage Gamma, corresponds to aa 3 ... 166, 57% identity)
• [AF|O34391]

Paralogous protein(s)

• [protein|C9C395A5F6A2C06EAC97F228C5C66D1628C937CF|cwlA], [protein|D5612882F1887BE342EF7072E71502382AB7289F|cwlH], [protein|50D7B97E4D3D41F74EF1942B85DE1DA4A33BEBB0|xlyA]

[wiki|Localization]

• secreted (according to UniProt)

Expression and Regulation

Operons

• genes: [gene|A8AF827637B7208DFA101C9A2911BF9AB1AED08F|xlyB]

  description: [pubmed|22383849]

additional information

• the mRNA is very stable (> 15 min) [pubmed|12884008]

Biological materials

Mutant

• BKE12460 ([gene|A8AF827637B7208DFA101C9A2911BF9AB1AED08F|xlyB]::erm  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE12460 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CACCTATCATCTCTCCTTTT,  downstream forward: _UP4_TAAATGAAAAGACGGAAGCT
• BKK12460 ([gene|A8AF827637B7208DFA101C9A2911BF9AB1AED08F|xlyB]::kan  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK12460 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CACCTATCATCTCTCCTTTT,  downstream forward: _UP4_TAAATGAAAAGACGGAAGCT

References

Reviews

• 18430080

Original Publications

• 12884008