vpr

168

minor extracellular serine protease

locus: BSU_38090

Molecular weight: 85.42 kDa

pI: 5.77

Protein length: 806 aa

Gene length: 2421 bp

function: protein degradation

product: minor extracellular serine protease

essential: no

synonyms: vpr, ipa-45r

List of homologs in different organisms, belongs to COG1404 (Galperin et al., 2021)

Gene

Coordinates: 3,907,844  3,910,264

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The protein

Protein family

• [wiki|peptidase S8 family] (according to UniProt)

[wiki|Domains]

• Inhibitor I9 domain (aa 57-142) (according to UniProt)
• Peptidase S8 domain (aa 158-579) (according to UniProt)

Structure

• [AF|P29141]

[wiki|Localization]

• extracellular (signal peptide) [Pubmed|18957862]
• forms enzymatically active fibrils outside the cell [pubmed|37980359]

Expression and Regulation

Operons

• genes: [gene|481AF959FDED9055F33524483101DA9AF2013151|vpr]

  description: [Pubmed|9353933]

  regulation

  • expressed under conditions of phosphate limitation ([protein|search|PhoP]) [Pubmed|16291680]

  regulatory mechanism

  • [protein|90ACE0DECA58D3C1A55E135120F7A0C1C920571C|codY]: repression, [Pubmed|25666135], in [regulon|protein:90ACE0DECA58D3C1A55E135120F7A0C1C920571C|codY regulon]
  • [protein|638D6D2FCCA2A167EFED05CB4E60C2D78D5319AE|phoP]: activation, [Pubmed|16291680], in [regulon|protein:638D6D2FCCA2A167EFED05CB4E60C2D78D5319AE|phoP regulon]
  • [protein|D267AF38B0CF107042326E9B8F3DD3C9C85840E4|lexA]: repression, [Pubmed|16267290], in [regulon|protein:D267AF38B0CF107042326E9B8F3DD3C9C85840E4|lexA regulon]
  • [protein|6740108089F13116F200C15F35C2E7561E990FEB|dnaA]: repression, [Pubmed|26020636], in [regulon|protein:6740108089F13116F200C15F35C2E7561E990FEB|dnaA regulon]

  sigma factors

  • [protein|DC3449D5F195E5C2E9E14FEC95396C8F1FDF73B4|sigH]: sigma factor, [Pubmed|25666135], in [regulon|protein:DC3449D5F195E5C2E9E14FEC95396C8F1FDF73B4|sigH regulon]

Biological materials

Mutant

• KO7 (''nprE  aprE  epr  mpr  nprB  vpr  bpr''), available as BGSC 1A1133
• BKE38090 ([gene|481AF959FDED9055F33524483101DA9AF2013151|vpr]::erm  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE38090 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CAATGTGTTTCCCCCTTTGT,  downstream forward: _UP4_TAAGAAAAAGCCCTGCCGAT
• BKK38090 ([gene|481AF959FDED9055F33524483101DA9AF2013151|vpr]::kan  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK38090 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CAATGTGTTTCCCCCTTTGT,  downstream forward: _UP4_TAAGAAAAAGCCCTGCCGAT

References

Reviews

• 34410368

Original Publications

• 24115457,16291680,16267290,18957862,25666135,26020636,1938892,37980359