ureA

168

urease (gamma subunit)

Locus: BSU_36660

Molecular weight: 11.32 kDa

Isoelectric point: 4.98

Protein length: 105 aa

Gene length: 318 bp

Function: utilization of urea as alternative nitrogen source

Product: urease (gamma subunit)

Essential: no

E.C.: 3.5.1.5

Synonyms: ureA

Genomic Context

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List of homologs in different organisms, belongs to COG0831 (Galperin et al., 2021)

Gene

Coordinates: 3,768,791  3,769,108

The protein

Catalyzed reaction/ biological activity

• 2 H⁺ + H₂O + urea --> CO₂ + 2 NH₄⁺ (according to UniProt)

Protein family

• urease gamma subunit family (single member, according to UniProt)

Structure

• 2FVH (PDB) (from Mycobacterium tuberculosis, 57% identity, 62% similarity)
• P75030 (AlphaFold)

Localization

• cytoplasm (according to Swiss-Prot)

Expression and Regulation

Operons

• Genes: ywzF-ywzE-ureA-ureB-ureC

  Description: PubMed

• Genes: ureA-ureB-ureC

  Description: PubMed

  Regulation

  • induced by nitrogen limitation (GlnR, TnrA) PubMed

  Regulatory mechanism

  • GlnR: repression, PubMed, in glnR regulon
  • TnrA: activation, PubMed, in tnrA regulon
  • PucR: activation, PubMed, in pucR regulon
  • CodY: repression, PubMed, in codY regulon

  Sigma factors

  • SigA: sigma factor, PubMed, in sigA regulon
  • SigH: sigma factor, PubMed, in sigH regulon

Biological materials

Mutant

• BKE36660 (ureA::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATCTGTAGTCCTCCTTCTT,  downstream forward: _UP4_CCAATTTCTGCGGAGGTGAA
• BKK36660 (ureA::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATCTGTAGTCCTCCTTCTT,  downstream forward: _UP4_CCAATTTCTGCGGAGGTGAA

References

Reviews

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Original Publications

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