tsaB

168

required for threonyl carbamoyl adenosine (t6A) modification of tRNAs that pair with ANN codons in mRNA

locus: BSU_05920

Molecular weight: 25.10 kDa

pI: 5.76

Protein length: 229 aa

Gene length: 690 bp

function: tRNA modification

product: tRNA modification enzyme

essential: yes

synonyms: tsaB, ydiC

List of homologs in different organisms, belongs to COG1214 (Galperin et al., 2021)

Gene

Coordinates: 642,111  642,800

Phenotypes of a mutant

• essential [Pubmed|12682299]

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The protein

Catalyzed reaction/ biological activity

• biosynthesis of the threonylcarbamoyladenosine (t6A) residue at position 37 of ANN-decoding tRNAs using L-threonylcarbamoyl-AMP ([protein|6CB350C335603D5F4434679F9D740BFECA738599|tsaB]-[protein|30393F16CFE89E7BAD233A0BD27B77D679F7E409|tsaD]-[protein|E15BB518649B2B9CD44CEBBB4FA481E55BCE823C|tsaE]) [Pubmed|23072323,21183954]

Protein family

• KAE1 / TsaD family (with [protein|30393F16CFE89E7BAD233A0BD27B77D679F7E409|tsaD], according to UniProt)

Structure

• [PDB|2A6A] (from ''Thermotoga maritima'', 24% identity) [Pubmed|20944216]
• [AF|O05516]

[wiki|Localization]

• cytoplasm (according to UniProt)

Expression and Regulation

Operons

• genes: [gene|DE32F1DA1EA1164411E0605262381BD38A49656C|thiL]-[gene|E15BB518649B2B9CD44CEBBB4FA481E55BCE823C|tsaE]-[gene|6CB350C335603D5F4434679F9D740BFECA738599|tsaB]-[gene|C2283BDD806221A285A27F24F52F92F4D47F0C55|rimI]-[gene|30393F16CFE89E7BAD233A0BD27B77D679F7E409|tsaD]

  description: [Pubmed|22383849]

References

• 19376873,17005971,21285948,23072323,22383849,20944216,29741707,33524148