truB

168

tRNA pseudouridine 55 synthase

locus: BSU_16660

Molecular weight: 34.05 kDa

pI: 6.65

Protein length: 309 aa

Gene length: 930 bp

function: tRNA modification

product: tRNA pseudouridine 55 synthase

essential: no

synonyms: truB, ylxN, ymxF

List of homologs in different organisms, belongs to COG0130 (Galperin et al., 2021)

Gene

Coordinates: 1,736,886  1,737,815

Advertisement

The protein

Catalyzed reaction/ biological activity

• uridine⁵⁵ in tRNA --> pseudouridine⁵⁵ in tRNA (according to UniProt)

Protein family

• pseudouridine synthase TruB family (single member, according to UniProt)

Structure

• [PDB|1ZE1] (from Thermotoga maritima, 36% identity) [pubmed|14990747]
• [AF|P32732]

Biological materials

Mutant

• BKE16660 ([gene|425FC9847D10A6FA9725D6523CD3213C57B371BE|truB]::erm  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE16660 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CATTTGCCTTTCACTCCTTC,  downstream forward: _UP4_CAATAGAAAAAAGGTGACCG
• BKK16660 ([gene|425FC9847D10A6FA9725D6523CD3213C57B371BE|truB]::kan  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK16660 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CATTTGCCTTTCACTCCTTC,  downstream forward: _UP4_CAATAGAAAAAAGGTGACCG

References

• 24371284,7489483,14990747