treP

168

trehalose permease of the phosphotransferase system, EIIBC of the PTS

Locus: BSU_07800

Molecular weight: 49.84 kDa

Isoelectric point: 8.56

Protein length: 470 aa

Gene length: 1413 bp

Function: trehalose uptake and phosphorylation

Product: trehalose-specific PTS permease, EIIBC component

Essential: no

E.C.: 2.7.1.69

Synonyms: treP, treB

Genomic Context

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Categories containing this gene/protein

Cellular processes, Transporters, Phosphotransferase system, Sugar specific PTS proteins

Metabolism, Carbon metabolism, Utilization of specific carbon sources, Utilization of trehalose

Groups of genes, Membrane proteins

Groups of genes, Phosphoproteins, Phosphorylation on a Cys residue

List of homologs in different organisms, belongs to COG1264 (Galperin et al., 2021)

Gene

Coordinates: 850,367 → 851,779

The protein

Catalyzed reaction/ biological activity

α,α-trehalose + N^π-phospho-L-histidyl-[protein] --> α,α-trehalose 6-phosphate + L-histidyl-[protein] (according to UniProt)

Protein family

PTS permease, sucrose family PubMed

Domains

PTS EIIB domain type-1 (aa 1-88) (according to UniProt)
PTS EIIC domain type-1 (aa 108-470) (according to UniProt)

Structure

3IPJ (PDB) (from Clostridium difficile, the PTS EIIB domain, 41.4% identity)
P39794 (AlphaFold)

Paralogous protein(s)

SacX, SacP, MurP, BglP

Localization

cell membrane (according to UniProt)

Expression and Regulation

Operons

Genes: treP-treA-treR

Description: PubMed

Regulation

expressed under conditions of phosphate limitation (PhoP) PubMed

Regulatory mechanism

TreR: repression, PubMed, in treR regulon
CcpA: repression, PubMed, in ccpA regulon
PhoP: activation, PubMed, in phoP regulon

Sigma factors

SigA: sigma factor, PubMed, in sigA regulon

Biological materials

Mutant

MGNA-C259 (treP::erm), available at the NBRP B. subtilis, Japan
BKE07800 (ΔtreP::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATGCCAACCCCTCCGTATC,  downstream forward: _UP4_TAACAAGTGGGGAGCGGGAC
BKK07800 (ΔtreP::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATGCCAACCCCTCCGTATC,  downstream forward: _UP4_TAACAAGTGGGGAGCGGGAC

References

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