tig

168

trigger factor (prolyl isomerase)

locus: BSU_28230

Molecular weight: 47.00 kDa

pI: 4

Protein length: 424 aa

Gene length: 1275 bp

function: protein folding

product: trigger factor (prolyl isomerase)

essential: no

ec: 5.2.1.8

synonyms: tig, yzzH

List of homologs in different organisms, belongs to COG0544 (Galperin et al., 2021)

Gene

Coordinates: 2,886,315 2,887,589

Phenotypes of a mutant

• delayed spore [wiki|germination] [Pubmed|25661487]
• a [gene|B6BF089BDC615205C9E4032380B1F0D5568D7C00|tig] [gene|30E0AAABB803E577D0D9FBAEF9031319CABD3D89|dnaK] double mutant exhibits an aberrant twisted and filamentous cell morphology, as well as decreased tolerance to heat and to cell wall active antibiotics and hydrolytic enzymes, indicative of defects in cell wall integrity [pubmed|36726573]

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The protein

Catalyzed reaction/ biological activity

• [protein]-peptidylproline (=180) --> [protein]-peptidylproline (=0) (according to UniProt)

Protein family

• FKBP-type PPIase family (single member, according to UniProt)

[wiki|Domains]

• PPIase FKBP-type domain (aa 163-248) (according to UniProt)

Structure

• [PDB|2VRH] (the ''E. coli'' trigger factor) [Pubmed|18497744]
• [AF|P80698]

Modification

• phosphorylated on Arg-45, the phosphorylation interferes with [protein|B6BF089BDC615205C9E4032380B1F0D5568D7C00|tig] binding to ribosomes [Pubmed|31221751]
• phosphorylated on Arg-90 [Pubmed|22517742]
• phosphorylated on ser/ thr/ tyr [Pubmed|16493705]

Additional information

• subject to Clp-dependent proteolysis upon glucose starvation [Pubmed|17981983]
• belongs to the 100 most abundant proteins [PubMed|15378759]

Expression and Regulation

Operons

• genes: [gene|B6BF089BDC615205C9E4032380B1F0D5568D7C00|tig]

  description: [Pubmed|8969504]

  regulation

  • [protein|search|RelA] dependent downregulation (Class I) during stringent response [Pubmed|11948165]

  regulatory mechanism

  • stringent response: negative regulation, in [regulon|other_regulator:stringent response|stringent response]

Biological materials

Mutant

• BKE28230 ([gene|B6BF089BDC615205C9E4032380B1F0D5568D7C00|tig]::erm trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE28230 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATGTGTTTCCCTCCAAAAA, downstream forward: _UP4_TAATATGGTGCATAATAATA
• BKK28230 ([gene|B6BF089BDC615205C9E4032380B1F0D5568D7C00|tig]::kan trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK28230 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATGTGTTTCCCTCCAAAAA, downstream forward: _UP4_TAATATGGTGCATAATAATA

References

Reviews

• 16231086,15763705,15837180,19647435,27890920,28724440

Original Publications

• 36815589,12226666,12224648,9748346,9063446,18497744,16493705,8969504,20525796,22517742,16271892,16091460,15378759,25661487,31221751,31740237,36726573