thyA

168

thymidylate synthase A

Locus: BSU_17680

Molecular weight: 32.65 kDa

Isoelectric point: 5.5

Protein length: 279 aa

Gene length: 840 bp

Function: biosynthesis of thymidine nucleotides

Product: thymidylate synthase A

Essential: no

E.C.: 2.1.1.45

Synonyms: thyA

Genomic Context

Hide small RNAsZoom:

Categories containing this gene/protein

Metabolism, Nucleotide metabolism, Biosynthesis/ acquisition of nucleotides, Biosynthesis/ acquisition of pyrimidine nucleotides

List of homologs in different organisms, belongs to COG0207 (Galperin et al., 2021)

Gene

Coordinates: 1,902,219  1,903,058

The protein

Catalyzed reaction/ biological activity

(6R)-5,10-methylene-5,6,7,8-tetrahydrofolate + dUMP --> 7,8-dihydrofolate + dTMP (according to UniProt)

Protein family

thymidylate synthase family (with ThyB, according to UniProt)

Structure

1B02 (PDB) PubMed
P0CI79 (AlphaFold)

Paralogous protein(s)

ThyB

Localization

cytoplasm (according to Swiss-Prot)

Expression and Regulation

Operons

Genes: thyA

Description: PubMed

Biological materials

Mutant

BKE17680 (thyA::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATGACTATTCATATCCTTC,  downstream forward: _UP4_TAATGCTGCCTTTTTATTGT
BKK17680 (thyA::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATGACTATTCATATCCTTC,  downstream forward: _UP4_TAATGCTGCCTTTTTATTGT

References

Reviews

Loading

Original Publications

Loading