thiS

168

sulfur carrier protein, hydroxyethylthiazole phosphate biosynthesis

locus: BSU_11680

Molecular weight: 7.49 kDa

pI: 5.5

Protein length: 66 aa

Gene length: 201 bp

function: biosynthesis of thiamine

product: sulfur carrier protein

essential: no

ec: null

synonyms: thiS, yjbS

List of homologs in different organisms, belongs to COG2104 (Galperin et al., 2021)

Gene

Coordinates: 1,244,844  1,245,044

Advertisement

The protein

Protein family

• sulfur carrier protein ThiS family (single member, according to UniProt)

Structure

• [PDB|1TYG] (complex with [protein|B58B8F260E446E28BC2E3A9AA84EA771EC0A8634|thiG]) [Pubmed|15362849]
• [AF|O31617]

Expression and Regulation

Operons

• genes: [gene|47E47FB7D9C079DF603906694C4649A049814346|tenA]-[gene|CA6AAE8AC5B5EF01A911B035F8782579CD876C08|tenI]-[gene|87B5C84B7EE10F60B545CCFEF6A527A546B80432|thiO]-[gene|95DA3F899037140F48B99E065C768356CE08FA94|thiS]-[gene|B58B8F260E446E28BC2E3A9AA84EA771EC0A8634|thiG]-[gene|CCD3DDE9E52FCD4906735059CF30593BEB56FD1B|thiF]-[gene|97EDB4ACD64C5FA2E6014ED133DF18F602FF58BB|thiD]

  description: [Pubmed|1898926]

  regulation

  • repressed by thiamine ([wiki|Thi-box]) [Pubmed|16356850]
  • the [wiki|Thi-box] RNA is degraded by [protein|872CCB5A49C9000BD95E4B0472556D5F60F7D7A4|RNase Y] [pubmed|29794222]

  regulatory mechanism

  • [protein|E195900D971AEE4A790D4579BE5E5A15B81010B8|Thi-box]: RNA switch, via [wiki|RNA switch], in [regulon|protein:E195900D971AEE4A790D4579BE5E5A15B81010B8|Thi-box regulon]

Biological materials

Mutant

• BKE11680 ([gene|95DA3F899037140F48B99E065C768356CE08FA94|thiS]::erm  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE11680 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_GTCTTTACCGTTCAGCTGTA,  downstream forward: _UP4_ATTGTCCATTTTGTAGGAGG
• BKK11680 ([gene|95DA3F899037140F48B99E065C768356CE08FA94|thiS]::kan  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK11680 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_GTCTTTACCGTTCAGCTGTA,  downstream forward: _UP4_ATTGTCCATTTTGTAGGAGG

References

Reviews

• 19348578,10382260

Original Publications

• 15362849,14567704