thiM

168

hydroxyethylthiazole kinase

locus: BSU_38300

Molecular weight: 28.06 kDa

pI: 5.39

Protein length: 272 aa

Gene length: 819 bp

function: biosynthesis of thiamine

product: hydroxyethylthiazole kinase

essential: no

ec: 2.7.1.50

synonyms: thiM, thiK, ipa-25d, ywbJ

List of homologs in different organisms, belongs to COG2145 (Galperin et al., 2021)

Gene

Coordinates: 3,931,372  3,932,190

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The protein

Catalyzed reaction/ biological activity

• 5-(2-hydroxyethyl)-4-methylthiazole + ATP --> 4-methyl-5-(2-phosphooxyethyl)-thiazole + ADP + H⁺ (according to UniProt)

Protein family

• Thz kinase family (single member, according to UniProt)

Structure

• [PDB|1C3Q]
• [AF|P39593]

Expression and Regulation

Operons

• genes: [gene|CC55120C2AF455FA2AEAE39E21F57897B2B5BC39|ywbI]-[gene|9B82059498F72A67282DD54892EF02A305E3C674|thiM]-[gene|8FAEB6342E087BEDC16FDF5585B03B90ACFF8D11|thiE]

  description: [Pubmed|9139923]

  sigma factors

  • [protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA]: sigma factor, [Pubmed|9139923], in [regulon|protein:360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA regulon]

Biological materials

Mutant

• BKE38300 ([gene|9B82059498F72A67282DD54892EF02A305E3C674|thiM]::erm  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE38300 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CATATTTGCATCATCCTTTA,  downstream forward: _UP4_TCATGACTCGTATTTCTCGG
• BKK38300 ([gene|9B82059498F72A67282DD54892EF02A305E3C674|thiM]::kan  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK38300 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CATATTTGCATCATCCTTTA,  downstream forward: _UP4_TCATGACTCGTATTTCTCGG

References

Reviews

• 19348578

Original Publications

• 10891066,9139923