thiI

168

sulfuryl transferase, biosynthesis of 4-thiouridine in tRNA

Locus: BSU_29580

Molecular weight: 40.17 kDa

Isoelectric point: 9.66

Protein length: 401 aa

Gene length: 1206 bp

Function: biosynthesis of 4-thiouridine in tRNA

Product: sulfuryl transferase

Essential: no

Synonyms: thiI, ytbJ

Genomic Context

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List of homologs in different organisms, belongs to COG0301 (Galperin et al., 2021)

Gene

Coordinates: 3,025,748  3,026,953

The protein

Catalyzed reaction/ biological activity

• receives a sulfuryl group from NifZ and transfers it to uridine  at position 8 of tRNA  PubMed
• [ThiI sulfur-carrier protein]-S-sulfanyl-L-cysteine +  uridine in tRNA + ATP + H⁺ + 2 reduced [2Fe-2S]-[ferredoxin] --> [ThiI sulfur-carrier protein]-L-cysteine +  4-thiouridine in tRNA + AMP + diphosphate + 2 oxidized [2Fe-2S]-[ferredoxin] (according to UniProt)
• [sulfur-carrier protein ThiS]-C-terminal Gly-Gly-AMP + AH₂ + S-sulfanyl-L-cysteinyl-[cysteine desulfurase] --> [sulfur-carrier protein ThiS]-C-terminal Gly-NH-CH₂-C(O)SH + A + AMP + H⁺ + L-cysteinyl-[cysteine desulfurase] (according to UniProt)

Protein family

• thiI family (single member, according to UniProt)

Domains

• THUMP domain (aa 60-165) (according to UniProt)

Structure

• 4KR6 (PDB) (ThiI-RNA complex from Thermotoga maritima) PubMed
• 2C5S (PDB) (from B. anthracis) PubMed
• O34595 (AlphaFold)

Localization

• cytoplasm (according to Swiss-Prot)

Expression and Regulation

Operons

• Genes: nifZ-thiI-sspA

  Description: PubMed

Biological materials

Mutant

• BKE29580 (thiI::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_TAATATATGATCGTAATTCA,  downstream forward: _UP4_TAAATCAATTTTCAGCTCCT
• BKK29580 (thiI::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_TAATATATGATCGTAATTCA,  downstream forward: _UP4_TAAATCAATTTTCAGCTCCT

References

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