tenI

168

thiazole tautomerase

Locus: BSU_11660

Molecular weight: 22.78 kDa

Isoelectric point: 8.13

Protein length: 205 aa

Gene length: 618 bp

Function: biosynthesis of thiamine

Product: thiazole tautomerase

Essential: no

E.C.: null

Synonyms: tenI

Genomic Context

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List of homologs in different organisms, belongs to COG0352 (Galperin et al., 2021)

Gene

Coordinates: 1,243,134 → 1,243,751

The protein

Catalyzed reaction/ biological activity

• 2-[(2R,5Z)-2-carboxy-4-methylthiazol-5(2H)-ylidene]ethyl phosphate --> 2-(2-carboxy-4-methylthiazol-5-yl)ethyl phosphate (according to UniProt)

Protein family

• thiazole tautomerase family (single member, according to UniProt)

Structure

• 1YAD (PDB)
• P25053 (AlphaFold)

Paralogous protein(s)

• ThiE

Expression and Regulation

Operons

• Genes: tenA-tenI-thiO-thiS-thiG-thiF-thiD

  Description: PubMed

  Regulation

  • repressed by thiamine (Thi-box) PubMed
  • antitermination is modulated by NusG-dependent transcription pausing just upstream of the Thi-box PubMed
  • the Thi-box RNA is degraded by RNase Y PubMed

  Regulatory mechanism

  • Thi-box: RNA switch, via RNA switch, in Thi-box regulon

Biological materials

Mutant

• BKE11660 (ΔtenI::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CGCTAGCTCTTCTACCGGCT,  downstream forward: _UP4_TCCCGCAAGCTAAAGGAGAT
• BKK11660 (ΔtenI::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CGCTAGCTCTTCTACCGGCT,  downstream forward: _UP4_TCCCGCAAGCTAAAGGAGAT

References

Reviews

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Original Publications

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