tenI

168

thiazole tautomerase

locus: BSU_11660

Molecular weight: 22.78 kDa

pI: 8.13

Protein length: 205 aa

Gene length: 618 bp

function: biosynthesis of thiamine

product: thiazole tautomerase

essential: no

ec: null

synonyms: tenI

List of homologs in different organisms, belongs to COG0352 (Galperin et al., 2021)

Gene

Coordinates: 1,243,134 → 1,243,751

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The protein

Catalyzed reaction/ biological activity

• 2-[(2R,5Z)-2-carboxy-4-methylthiazol-5(2H)-ylidene]ethyl phosphate --> 2-(2-carboxy-4-methylthiazol-5-yl)ethyl phosphate (according to UniProt)

Protein family

• thiazole tautomerase family (single member, according to UniProt)

Structure

• [PDB|1YAD]
• [AF|P25053]

Paralogous protein(s)

• [protein|8FAEB6342E087BEDC16FDF5585B03B90ACFF8D11|thiE]

Expression and Regulation

Operons

• genes: [gene|47E47FB7D9C079DF603906694C4649A049814346|tenA]-[gene|CA6AAE8AC5B5EF01A911B035F8782579CD876C08|tenI]-[gene|87B5C84B7EE10F60B545CCFEF6A527A546B80432|thiO]-[gene|95DA3F899037140F48B99E065C768356CE08FA94|thiS]-[gene|B58B8F260E446E28BC2E3A9AA84EA771EC0A8634|thiG]-[gene|CCD3DDE9E52FCD4906735059CF30593BEB56FD1B|thiF]-[gene|97EDB4ACD64C5FA2E6014ED133DF18F602FF58BB|thiD]

  description: [Pubmed|1898926]

  regulation

  • repressed by thiamine ([wiki|Thi-box]) [Pubmed|16356850]
  • the [wiki|Thi-box] RNA is degraded by [protein|872CCB5A49C9000BD95E4B0472556D5F60F7D7A4|RNase Y] [pubmed|29794222]

  regulatory mechanism

  • [protein|E195900D971AEE4A790D4579BE5E5A15B81010B8|Thi-box]: RNA switch, via [wiki|RNA switch], in [regulon|protein:E195900D971AEE4A790D4579BE5E5A15B81010B8|Thi-box regulon]

Biological materials

Mutant

• BKE11660 (Δ[gene|CA6AAE8AC5B5EF01A911B035F8782579CD876C08|tenI]::erm  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE11660 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CGCTAGCTCTTCTACCGGCT,  downstream forward: _UP4_TCCCGCAAGCTAAAGGAGAT
• BKK11660 (Δ[gene|CA6AAE8AC5B5EF01A911B035F8782579CD876C08|tenI]::kan  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK11660 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CGCTAGCTCTTCTACCGGCT,  downstream forward: _UP4_TCCCGCAAGCTAAAGGAGAT

References

Reviews

• 10382260,22616866

Original Publications

• 15709744,1898926,21534620