tdk

168

thymidine kinase

locus: BSU_37060

Molecular weight: 21.30 kDa

pI: 5.69

Protein length: 195 aa

Gene length: 588 bp

function: biosynthesis of thymidine nucleotides

product: thymidine kinase (ATP:thymidine)

essential: no

ec: 2.7.1.21

synonyms: tdk

List of homologs in different organisms, belongs to COG1435 (Galperin et al., 2021)

Gene

Coordinates: 3,802,405 → 3,802,992

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The protein

Catalyzed reaction/ biological activity

• ATP + thymidine --> ADP + dTMP + H⁺ (according to UniProt)

Protein family

• thymidine kinase family (single member, according to UniProt)

Structure

• [PDB|2JA1] (from B. cereus, 69% identity) [pubmed|17288553]
• [AF|Q03221]

[wiki|Localization]

• cytoplasm (according to Swiss-Prot)

Biological materials

Mutant

• BKE37060 (Δ[gene|D97D4CFDF7A9A48A8764738ECFD42F1D3A900BE7|tdk]::erm  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE37060 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CATAGGCTTCGCTCCCCTCT,  downstream forward: _UP4_TAGTCCAAAACATGGGTATA
• BKK37060 (Δ[gene|D97D4CFDF7A9A48A8764738ECFD42F1D3A900BE7|tdk]::kan  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK37060 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CATAGGCTTCGCTCCCCTCT,  downstream forward: _UP4_TAGTCCAAAACATGGGTATA

References

Research papers

• 17288553