tdh

168

L-threonine dehydrogenase

locus: BSU_16990

Molecular weight: 36.84 kDa

pI: 6.06

Protein length: 347 aa

Gene length: 1044 bp

function: threonine utilization

product: L-threonine dehydrogenase

essential: no

ec: 1.1.1.103

synonyms: tdh

List of homologs in different organisms, belongs to COG1063 (Galperin et al., 2021)

Gene

Coordinates: 1,770,461  1,771,504

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The protein

Catalyzed reaction/ biological activity

• L-threonine + NAD⁺ --> (2S)-2-amino-3-oxobutanoate + H⁺ + NADH (according to UniProt)

Protein family

• [wiki|zinc-containing alcohol dehydrogenase family] (according to UniProt)

[wiki|Cofactors]

• NAD⁺ (according to UniProt)

Structure

• [PDB|2DQ4] (from ''Thermus thermophilus'', 47% identity, 63% similarity)
• [AF|O31776]

[wiki|Localization]

• cytoplasm (according to Swiss-Prot)

Biological materials

Mutant

• BKE16990 ([gene|8E64B62F15B92A2AACD68B913909100AE276D114|tdh]::erm  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE16990 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CATGTACAATCCTCCTTGAA,  downstream forward: _UP4_TTAATTCCATAAAGGGGGAT
• BKK16990 ([gene|8E64B62F15B92A2AACD68B913909100AE276D114|tdh]::kan  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK16990 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CATGTACAATCCTCCTTGAA,  downstream forward: _UP4_TTAATTCCATAAAGGGGGAT

References

Research papers

• 31585995