tcyA

168

cystine and diaminopimelate ABC transporter (binding protein)

Locus: BSU_03610

Molecular weight: 29.32 kDa

Isoelectric point: 9.12

Protein length: 268 aa

Gene length: 807 bp

Function: cystine uptake

Product: cystine and diaminopimelate ABC transporter (binding protein)

Essential: no

Synonyms: tcyA, yckK

Genomic Context

Hide small RNAsZoom:

List of homologs in different organisms, belongs to COG0834 (Galperin et al., 2021)

Gene

Coordinates: 410,656  411,462

The protein

Catalyzed reaction/ biological activity

• uptake of cystine and diaminopimelate PubMed

Protein family

• bacterial solute-binding protein 3 family (according to UniProt)

Structure

• 2YLN (PDB) (from Neisseria gonorrhoeae, 54% identity) PubMed
• P42199 (AlphaFold)

Paralogous protein(s)

• YckB

Localization

• attached to the membrane via TcyB PubMed

Expression and Regulation

Operons

• Genes: tcyA-tcyB-tcyC

  Description: PubMed

  Regulation

  • strongly repressed in response to glucose starvation in M9 medium PubMed

Biological materials

Mutant

• GP4658 (trpC2 ΔtcyA::neo), available in Jörg Stülke's lab
• GP4667 (trpC2 ΔtcyA::lox72), available in Jörg Stülke's lab
• BKE03610 (tcyA::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATCTTATTCCCCACTTTTC,  downstream forward: _UP4_GAAGATGTTTCTAAATAATC
• BKK03610 (tcyA::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATCTTATTCCCCACTTTTC,  downstream forward: _UP4_GAAGATGTTTCTAAATAATC

References

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