tagV

168

phosphotransferase, attaches teichoic acid, teichuronic acid, or acidic capsular polysaccharide to cell wall peptidoglycan via phosphodiester linkage to N-acteylmuramic acid

locus: BSU_35520

Molecular weight: 43.05 kDa

pI: 9.14

Protein length: 391 aa

Gene length: 1176 bp

function: transfer of anionic cell wall polymers from lipid-linked precursors to peptidoglycan

product: phosphotransferase, responsible for attachment of anionic polymers to peptidoglycan

essential: no

synonyms: tagV, yvhJ

List of homologs in different organisms, belongs to COG1316 (Galperin et al., 2021)

Gene

Coordinates: 3,647,406 → 3,648,581

Phenotypes of a mutant

• a ''[gene|E244A5ABB2FBD4FC3880F0DB700BD935991AE6DD|tagV]'' mutant has no phenoype, the triple ''[gene|68FE85BAA457199D34C9068F38756972CD2D280D|tagT] [gene|62A3CC548E033A45F94FE4386EE29711A6A14C6B|tagU] [gene|E244A5ABB2FBD4FC3880F0DB700BD935991AE6DD|tagV]'' mutant is unable to grow under normal conditions [Pubmed|21964069]

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The protein

Protein family

• LytR/CpsA/Psr (LCP) family (with [protein|62A3CC548E033A45F94FE4386EE29711A6A14C6B|tagU] and [protein|68FE85BAA457199D34C9068F38756972CD2D280D|tagT], according to UniProt) [Pubmed|19099556]

[wiki|Cofactors]

• Mg²⁺ [Pubmed|29107701,21964069]

Structure

• [PDB|6UF3] (aa 72-332) [pubmed|31969390]
• [PDB|3NXH]
• [AF|P96499]

Paralogous protein(s)

• [protein|62A3CC548E033A45F94FE4386EE29711A6A14C6B|tagU], [protein|68FE85BAA457199D34C9068F38756972CD2D280D|tagT]

[wiki|Localization]

• cytoplasmic membrane (with extracytoplasmic catalaytic domain) [Pubmed|21964069]

Expression and Regulation

Operons

• genes: [gene|8F46DEAD6195B566E1A5FDC6E6ED9D29969F4AFA|yvyE]-[gene|E244A5ABB2FBD4FC3880F0DB700BD935991AE6DD|tagV]

  description: [Pubmed|22383849]

  regulation

  • repressed under conditions that trigger sporulation ([wiki|Spo0A]) [Pubmed|14651647]

  regulatory mechanism

  • [protein|2C54FE2ADC82FF414D732018C90649D477A925AD|spo0A]: repression, [Pubmed|14651647], in [regulon|protein:2C54FE2ADC82FF414D732018C90649D477A925AD|spo0A regulon]

Biological materials

Mutant

• MGNA-A344 (yvhJ::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/344 NBRP B. subtilis, Japan]
• BKE35520 (Δ[gene|E244A5ABB2FBD4FC3880F0DB700BD935991AE6DD|tagV]::erm  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE35520 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_ACGCACTCTAACGCGTTCAG,  downstream forward: _UP4_TAAAAAATGCCCGGTCCTTT
• BKK35520 (Δ[gene|E244A5ABB2FBD4FC3880F0DB700BD935991AE6DD|tagV]::kan  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK35520 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_ACGCACTCTAACGCGTTCAG,  downstream forward: _UP4_TAAAAAATGCCCGGTCCTTT

References

Reviews

• 24024634

Original Publications

• 14651647,19099556,21964069,22383849,29107701,31969390