tagE

168

UDP-glucose:polyglycerol phosphate glucosyltransferase

locus: BSU_35730

Molecular weight: 78.12 kDa

pI: 6.03

Protein length: 673 aa

Gene length: 2022 bp

function: biosynthesis of teichoic acid

product: UDP-glucose:polyglycerol phosphate glucosyltransferase

essential: no

ec: 2.4.1.52

synonyms: tagE, rodD, gtaA, gtaD

List of homologs in different organisms, belongs to COG0438 (Galperin et al., 2021)

Gene

Coordinates: 3,678,399  3,680,420

Phenotypes of a mutant

• inactivation of ''tagE'' is advantageous in environments with fluctuating glucose availability [Pubmed|23924781]
• resistant to phage SPO1 [pubmed|30811056]

additional information

• the gene readily acquires mutations during cultivation with Arabidopsis thaliana roots [pubmed|37768063]

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The protein

Catalyzed reaction/ biological activity

• transfer of glucose from UDP-glucose to glycerol phosphate in polymeric form [Pubmed|21558268]
• 4-O-{[(2R)-1-glycerylphospho]_(n)-(2R)-1-glycerylphospho}-N-acetyl-β-D-mannosaminyl-(1→4)-N-acetyl-α-D-glucosaminyl undecaprenyl diphosphate + n UDP-α-D-glucose --> 4-O-{[(2R)-2-α-D-glucosyl-1-glycerylphospho]_(n)-(2R)-1-glycerylphospho}-N-acetyl-β-D-mannosaminyl-(1→4)-N-acetyl-α-D-glucosaminyl undecaprenyl diphosphate + n H⁺ + n UDP (according to UniProt)

Protein family

• [wiki|glycosyltransferase 1 family] (according to UniProt)
• [wiki|Glycosyltransferase 4 subfamily] (according to UniProt)

Structure

• [PDB|4X7R] (from Staphylococcus aureus, 26% identity) [pubmed|25624472]
• [AF|P13484]

Modification

• phosphorylation on Ser-2 [Pubmed|17218307]

[wiki|Localization]

• cytoplasm (according to Swiss-Prot)

Expression and Regulation

Operons

• genes: [gene|8620DA76A1953D7ACDFA7E46ED81EF1A0D97999C|tagD]-[gene|88028A5F3520CB6DAB014412974EB59CC5D226DD|tagE]-[gene|E730E41546298960A4B907FCB7F36C495AA9602B|tagF]-[gene|6795F1703416DCD04E51C177F1C9FE45E73ABAEF|tagG]-[gene|4D3C316965834130BB378CBD1265CFA13F086C7A|tagH]

  description: [Pubmed|22383849]

  regulation

  • repressed by phosphate ([protein|search|PhoP]) [Pubmed|9457886]

  regulatory mechanism

  • [protein|638D6D2FCCA2A167EFED05CB4E60C2D78D5319AE|phoP]: repression, [Pubmed|9457886], in [regulon|protein:638D6D2FCCA2A167EFED05CB4E60C2D78D5319AE|phoP regulon]
  • [protein|7F340423A34CE40D1F1AA8D373F7C4B859A6496D|walR]: activation, [Pubmed|12950927], in [regulon|protein:7F340423A34CE40D1F1AA8D373F7C4B859A6496D|walR regulon]

  sigma factors

  • [protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA]: sigma factor, [Pubmed|7581998], in [regulon|protein:360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA regulon]

Biological materials

Mutant

• 1A981 ( ''tagE''::''erm''), [Pubmed|18487323], available at [http://pasture.asc.ohio-state.edu/BGSC/getdetail.cfm?bgscid=1A981&Search=1A981 BGSC]
• BKE35730 ([gene|88028A5F3520CB6DAB014412974EB59CC5D226DD|tagE]::erm  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE35730 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CAATTTTTTACTCCCTTTCG,  downstream forward: _UP4_GTCACGGAAATAAAAGAGAG
• BKK35730 ([gene|88028A5F3520CB6DAB014412974EB59CC5D226DD|tagE]::kan  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK35730 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CAATTTTTTACTCCCTTTCG,  downstream forward: _UP4_GTCACGGAAATAAAAGAGAG

References

Reviews

• 24024634

Original Publications

• 9457886,7581998,2507871,17218307,12950927,23924781,21558268,22383849,25624472,30811056,37768063