swrAA/2

168

control of [protein|D343D2096664A972026D911E7A17A35C7B1CD1C9|degU] activity, enhances [gene|7024E4162A6D827069F882FDEACA696EBC05DD40|sigD] transcription, part of the [gene|5D479874B43F521DB52EDC2C27CDE4967F22DE47|swrA] pseudogene

locus: BSU_35239

Protein length: 38 aa

Gene length: 117 bp

function: essential for swarming differentiation on solid surfaces

product: swarming motility protein

essential: no

synonyms: swrAA/2

Gene

Coordinates: 3,621,931  3,622,047

additional information

• in the laboratory strain 168, the [gene|5D479874B43F521DB52EDC2C27CDE4967F22DE47|swrA] gene contains a frameshift mutation (insertion of an extra A in a stretch of eight As), thus the gene is annotated as [gene|0CEE58D799AF41634D161DBDF3D67EEFFF1C861E|swrAA/2] (corresponds to the small N-terminal part of the protein) and [gene|5D479874B43F521DB52EDC2C27CDE4967F22DE47|swrA] (corresponds to the large C-terminal part of the protein) [pubmed|15066026]
• the mutation readily reverts giving rise to swarming behaviour [pubmed|15066026]
• the [gene|5D479874B43F521DB52EDC2C27CDE4967F22DE47|swrA] gene is intact in wild type strains such as NCIB3610 [pubmed|15066026]

Biological materials

Mutant

• BKE35239 ([gene|0CEE58D799AF41634D161DBDF3D67EEFFF1C861E|swrAA/2]::erm  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE35239 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CATTCTCGCTAACGTTACTC,  downstream forward: _UP4_GGTTCACAATTGAAGAGGGC
• BKK35239 ([gene|0CEE58D799AF41634D161DBDF3D67EEFFF1C861E|swrAA/2]::kan  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK35239 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CATTCTCGCTAACGTTACTC,  downstream forward: _UP4_GGTTCACAATTGAAGAGGGC

labs

• see [protein|5D479874B43F521DB52EDC2C27CDE4967F22DE47|swrA]

References

• see [protein|5D479874B43F521DB52EDC2C27CDE4967F22DE47|swrA]